GapMind for catabolism of small carbon sources

 

Aligments for a candidate for opuBA in Pseudomonas fluorescens FW300-N2E2

Align BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) (characterized)
to candidate Pf6N2E2_3333 L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1)

Query= TCDB::Q93A35
         (328 letters)



>lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3333 L-proline glycine
           betaine ABC transport system permease protein ProV (TC
           3.A.1.12.1)
          Length = 385

 Score =  256 bits (653), Expect = 8e-73
 Identities = 127/275 (46%), Positives = 181/275 (65%), Gaps = 15/275 (5%)

Query: 1   MIRFDNVSKKYSDD--KTAAVNNVTLDIKDGEFFVFIGPSGCGKTTTLKMINRLIPLTTG 58
           MI   N+SK +  +     AV++V L + +GE  VF+GPSGCGK+TTLKMINRLIP T+G
Sbjct: 1   MIELQNLSKTFKSNGKDVKAVDSVNLTVNEGEICVFLGPSGCGKSTTLKMINRLIPPTSG 60

Query: 59  TIYINEKRISDYDIHELRWDIGYVLQQIALFPHMTIEENIAIVPELKKWSKEKIHDRITE 118
            + IN +  +  D   LR +IGYV+QQI LFP+MTIEENI +VP L  W K+K HDR  E
Sbjct: 61  KVLINGEDTTGLDEVTLRRNIGYVIQQIGLFPNMTIEENITVVPRLLGWDKQKCHDRARE 120

Query: 119 LLDSVGLDPESYRHRKPAELSGGEQQRVGVVRALAADPGIILMDEPFSALDPISRQRLQQ 178
           L+  + L+P+ Y HR P ELSGG+QQR+GV+RALAAD  ++LMDEPF A+DPI+R+ +Q 
Sbjct: 121 LMSMIKLEPKQYLHRYPRELSGGQQQRIGVIRALAADAPLLLMDEPFGAVDPINREMIQN 180

Query: 179 DISALQKKIKKTIVFVTHDMQEALALGDRICVMQGGEIVQVATPQEIMKNPENDFVKDFL 238
           +   +Q+ + KT++ V+HD+ EA+ LGD+I + + G++VQ   P  ++ +P ++FV  F+
Sbjct: 181 EFFEMQRALNKTVIMVSHDIDEAIKLGDKIAIFRAGKLVQCDHPDTLLAHPADEFVSSFV 240

Query: 239 -------------ASGHAFNTPILEANFTVNDLIE 260
                        A   A N P +     VN+ +E
Sbjct: 241 GQDSTLKRLLLVKAEDAADNAPSVSPETPVNEALE 275


Lambda     K      H
   0.318    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 307
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 328
Length of database: 385
Length adjustment: 29
Effective length of query: 299
Effective length of database: 356
Effective search space:   106444
Effective search space used:   106444
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory