Alignments for a candidate for treEIIA in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for treEIIA in Pseudomonas fluorescens FW300-N2E2

Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate Pf6N2E2_3942 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated

Query= reanno::pseudo3_N2E3:AO353_15995
         (844 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3942
          Length = 759

 Score =  299 bits (765), Expect = 5e-85
 Identities = 205/617 (33%), Positives = 319/617 (51%), Gaps = 35/617 (5%)

Query: 236 EDAELAISTLAQLLADGCGEAVTPVAVVAPVVEAQEVSTKLLRGVCASAGSAFGY-VVQV 294
           E  E  + T++  LA     A    ++     + + +      GV  S G+A G  VV +
Sbjct: 137 EGEEAFLVTMSAQLAGVIAHAEATGSISGLGRQGKGIQEAKFVGVPGSPGAAVGTAVVML 196

Query: 295 AERTLEM------PEFAADQQLERESLE--RALMHATQALQRLRDNAAGEAQADIFKAHQ 346
               L++       +  A+  L + ++E  RA M A  A  +L      E +A +F  + 
Sbjct: 197 PPADLDVVPDKAITDIDAELGLFKTAIEGVRADMRALSA--KLATQLRPEERA-LFDVYL 253

Query: 347 ELLEDPSLLEQAQALIAEGKSAAFAWNSATEATATLFKSLGSTLLAERALDLMDVGQRVL 406
            +L+D SL  +   +I  G+ A  A           F+ +    L ERA D+ D+G+R+L
Sbjct: 254 MMLDDASLGSEVTTVIKTGQWAQGALRQVVTDHVNRFELMDDAYLRERASDVKDLGRRLL 313

Query: 407 KLILGVPDGVWELPDQAILIAEQLTPSQTAALDTGKVLGFATVGGGATSHVAILARALGL 466
             +          PD  IL++E+LTP+    +  GK+ G  +V G   SHVAILARA+G+
Sbjct: 314 AYLQQERQQTLVYPDNTILVSEELTPAMLGEVPEGKLAGLVSVLGSGNSHVAILARAMGI 373

Query: 467 PAVCGLPLQVLSLASGTRVLLDADKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAAR 526
           P V GL     S   G ++++D   GE++ +P+  + +Q     ++++Q           
Sbjct: 374 PTVMGLVDLPYSKVDGIQMIVDGYHGEVYTNPSDVLRKQFADVVEEEKQLSLGLDALRDL 433

Query: 527 AAVTRDGHHFEVTANVASLAETEQAMSLGAEGIGLLRSEFLYQQRSVAPSHDEQAGTYSA 586
             VT DGH   +  N   LA+  +A   GAEG+GL R+E  +      PS  EQ   Y  
Sbjct: 434 PCVTLDGHRMPLWVNTGLLADVARAQKRGAEGVGLYRTEVPFMINQRFPSEKEQLAIYRE 493

Query: 587 IARALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAIL 646
              A  PQ  + +R+LD+GGDK L+Y P+  E NPFLG RGIR+ L+ P++   Q RA+L
Sbjct: 494 QLAAFHPQP-VTMRSLDIGGDKSLSYFPI-KEDNPFLGWRGIRVTLDHPEIFLVQARAML 551

Query: 647 -SSAGLARLHIMLPMVSQLSELRLARLMLEEEALALGLRE------------LPKLGIMI 693
            +S GL  L I+LPM+S   EL        EEAL L  R             +P +G+MI
Sbjct: 552 KASEGLNNLRILLPMISGTHEL--------EEALHLIHRAWGEVRDEGTDVPMPPIGVMI 603

Query: 694 EVPAAALMADLFAPEVDFFSIGTNDLTQYTLAMDRDHPRLASQADSFHPSVLRLIASTVK 753
           E+PAA       A +VDF S+G+NDLTQY LA+DR++PR+A   D  HP+VL+ + + V+
Sbjct: 604 EIPAAVYQTKELARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYDYLHPAVLQALQNVVR 663

Query: 754 AAHAHGKWVGVCGALASETLAVPLLLGLGVDELSVSVPLIPAIKAAIREVELSDCQAIAH 813
            AHA GK V +CG +A +  A  LL+ +G D LS++   +P +K  +R++ LS  Q +  
Sbjct: 664 DAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWMLRQINLSKAQELLA 723

Query: 814 QVLGLESAEQVREALSV 830
           +V+ +++ + +  +L +
Sbjct: 724 EVMTIDNPQVIHSSLQL 740


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1268
Number of extensions: 58
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 844
Length of database: 759
Length adjustment: 41
Effective length of query: 803
Effective length of database: 718
Effective search space:   576554
Effective search space used:   576554
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources