Alignments for a candidate for aacS in Pseudomonas fluorescens FW300-N2E2

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Pseudomonas fluorescens FW300-N2E2

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate Pf6N2E2_2190 Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2190
          Length = 565

 Score =  629 bits (1622), Expect = 0.0
 Identities = 311/559 (55%), Positives = 401/559 (71%), Gaps = 11/559 (1%)

Query: 16  SHARGATDVPLIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLG 75
           S+ RG+    L+ QTIG  F   +AR PE EALV  HQ  RYT+ QL       A ALL 
Sbjct: 11  SYTRGSAAKALLAQTIGEVFDQTMARYPEGEALVVRHQSLRYTWRQLAEAVDLHARALLA 70

Query: 76  MGLTPGDRVGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSM 135
           +GL  GDR+G+W+ N A+W + Q A+A++G++LVNINPAYR +E+EY L + GC+ LV  
Sbjct: 71  LGLKTGDRLGVWAPNCAQWCISQFASAKLGVILVNINPAYRVSELEYVLKQSGCQWLVCA 130

Query: 136 ARFKTSDYLGMLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTEL 195
             FKTSDY  ML+ LAPE   Q  G +Q+ +LP+L+ V+ +D +   G      L +++L
Sbjct: 131 GAFKTSDYHAMLQTLAPELAEQPIGQMQSERLPELRGVISLDSQPPSG-----FLPWSQL 185

Query: 196 IARGNAADP-RLAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKL 254
            A G A  P +LA+  + L    P+NIQ+TSGTTGFPKGATL+H NILNNG+ +GE + L
Sbjct: 186 AALGAAVTPGQLAERQSSLHFDQPVNIQYTSGTTGFPKGATLSHHNILNNGYMVGESLGL 245

Query: 255 TPADRLCIPVPLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVP 314
           T  DRL IPVPLYHCFGMV+GNL C THG+T++YPND FDPL  L+ V +E+ T L+GVP
Sbjct: 246 TADDRLVIPVPLYHCFGMVMGNLGCMTHGSTMIYPNDAFDPLLTLKAVAEEKATALYGVP 305

Query: 315 TMFIAELDHPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVS 374
           TMFIA LD P+  +F+LS+LRTGIMAG+ CP EVM+RV+ +M++ E+ IAYGMTETSPVS
Sbjct: 306 TMFIALLDQPQRGDFDLSSLRTGIMAGATCPIEVMRRVINEMHMAEVQIAYGMTETSPVS 365

Query: 375 CQSSTDTPLSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEA 434
            Q+     L  RV+TVG+ QP LE KI+D   G +VP G  GE CT+GYSVM GYW +  
Sbjct: 366 LQTGPTDELELRVTTVGRTQPQLESKIIDA-AGNLVPRGAVGELCTRGYSVMLGYWNNPK 424

Query: 435 KTREAIDEGGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQD 494
            T +AID  GWMHTGDLATMD +GYV IVGR KDM+IRGGENIYPRE+EEF + HP V D
Sbjct: 425 GTADAIDPDGWMHTGDLATMDEQGYVRIVGRNKDMIIRGGENIYPRELEEFFFTHPAVAD 484

Query: 495 VQVVGVPDQKYGEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVT 554
           VQV+G+P  +YGEE+ AWI   PG    E +++A+CK +IAH+K PR+ +FV  FPMTVT
Sbjct: 485 VQVIGIPCSRYGEEIVAWIKFHPGHSACEQELQAWCKERIAHFKTPRHFKFVEEFPMTVT 544

Query: 555 GKIQKFKIR----DEMKDQ 569
           GKIQKF++R    +E++D+
Sbjct: 545 GKIQKFRMREISIEELRDK 563


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 920
Number of extensions: 41
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 565
Length adjustment: 36
Effective length of query: 542
Effective length of database: 529
Effective search space:   286718
Effective search space used:   286718
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources