Align Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (characterized)
to candidate Pf6N2E2_479 Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)
Query= reanno::pseudo6_N2E2:Pf6N2E2_479 (423 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_479 Length = 423 Score = 821 bits (2120), Expect = 0.0 Identities = 423/423 (100%), Positives = 423/423 (100%) Query: 1 MGTHVIKMPDIGEGIAEVELSVWHVKVGDMVVEDQVLADVMTDKAMVDIPSPVHGRVIAL 60 MGTHVIKMPDIGEGIAEVELSVWHVKVGDMVVEDQVLADVMTDKAMVDIPSPVHGRVIAL Sbjct: 1 MGTHVIKMPDIGEGIAEVELSVWHVKVGDMVVEDQVLADVMTDKAMVDIPSPVHGRVIAL 60 Query: 61 GGEPGEVMAVGSELIRIEVEGAGNLKESAQQAPTPTPAAQAPKPAPVATPEPVLEKTAAP 120 GGEPGEVMAVGSELIRIEVEGAGNLKESAQQAPTPTPAAQAPKPAPVATPEPVLEKTAAP Sbjct: 61 GGEPGEVMAVGSELIRIEVEGAGNLKESAQQAPTPTPAAQAPKPAPVATPEPVLEKTAAP 120 Query: 121 RCAPQAPVARDPDERPLASPAVRKHALDLGIQLRLVQGSGPAGRVLHEDLEAYLAQGPSV 180 RCAPQAPVARDPDERPLASPAVRKHALDLGIQLRLVQGSGPAGRVLHEDLEAYLAQGPSV Sbjct: 121 RCAPQAPVARDPDERPLASPAVRKHALDLGIQLRLVQGSGPAGRVLHEDLEAYLAQGPSV 180 Query: 181 QAKGGSGYAERHDEQQIPVIGMRRKIAQRMQEATQRAAHFSYVEEIDITALEELRVHLNE 240 QAKGGSGYAERHDEQQIPVIGMRRKIAQRMQEATQRAAHFSYVEEIDITALEELRVHLNE Sbjct: 181 QAKGGSGYAERHDEQQIPVIGMRRKIAQRMQEATQRAAHFSYVEEIDITALEELRVHLNE 240 Query: 241 KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVIHRSGAVHVGVATQSDVGLMV 300 KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVIHRSGAVHVGVATQSDVGLMV Sbjct: 241 KHGASRGKLTLLPFLVRALVVALRDFPQMNARYDDEAQVIHRSGAVHVGVATQSDVGLMV 300 Query: 301 PVVRHAEARSLWDNAAEISRLATAARTGKASRDELSGSTITLTSLGALGGIVSTPVLNLP 360 PVVRHAEARSLWDNAAEISRLATAARTGKASRDELSGSTITLTSLGALGGIVSTPVLNLP Sbjct: 301 PVVRHAEARSLWDNAAEISRLATAARTGKASRDELSGSTITLTSLGALGGIVSTPVLNLP 360 Query: 361 EVAIVGVNKIVERPVVIKGQIVIRKMMNLSSSFDHRVVDGMDAAQFIQALRGLLEQPATL 420 EVAIVGVNKIVERPVVIKGQIVIRKMMNLSSSFDHRVVDGMDAAQFIQALRGLLEQPATL Sbjct: 361 EVAIVGVNKIVERPVVIKGQIVIRKMMNLSSSFDHRVVDGMDAAQFIQALRGLLEQPATL 420 Query: 421 FVE 423 FVE Sbjct: 421 FVE 423 Lambda K H 0.317 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 741 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 423 Length of database: 423 Length adjustment: 32 Effective length of query: 391 Effective length of database: 391 Effective search space: 152881 Effective search space used: 152881 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory