Align malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized)
to candidate Pf6N2E2_3462 Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)
Query= BRENDA::A0A081YAY7 (498 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 Length = 497 Score = 908 bits (2346), Expect = 0.0 Identities = 445/496 (89%), Positives = 467/496 (94%) Query: 1 MTLIKHLIGGELIADTGRTADVFNPSTGEAVRKVPLADRETMQQAIDAAKAAFPAWRNTP 60 M+LI HLI GEL++D+ RTADVFNPSTG+A+ KVPLADR T+QQAIDAAKAAFPAWRNTP Sbjct: 1 MSLIPHLINGELLSDSARTADVFNPSTGQAIHKVPLADRATIQQAIDAAKAAFPAWRNTP 60 Query: 61 PAKRAQVLFRFKQLLEANEERIVKLISEEHGKTIEDAAGELKRGIENVEYATAAPEILKG 120 AKRAQV+FRFKQLLE NE RI +LISEEHGKT+EDAAGELKRGIENVE+A AAPEILKG Sbjct: 61 AAKRAQVMFRFKQLLEQNESRIAQLISEEHGKTLEDAAGELKRGIENVEFACAAPEILKG 120 Query: 121 EYSRNVGPNIDAWSDFQPIGVVAGITPFNFPAMVPLWMYPLAIACGNTFILKPSERDPSS 180 EYSRNVGPNIDAWSDFQP+GVVAGITPFNFPAMVPLWMYPLAI CGN FILKPSERDPSS Sbjct: 121 EYSRNVGPNIDAWSDFQPLGVVAGITPFNFPAMVPLWMYPLAIVCGNCFILKPSERDPSS 180 Query: 181 TLLIAELFHEAGLPKGVLNVVHGDKGAVDALIEAPEVKALSFVGSTPIAEYIYSEGTKRG 240 TLLIA+L EAGLPKGVL+VVHGDK AVDALIEAPEVKALSFVGSTPIAEYIY+EGT+RG Sbjct: 181 TLLIAQLLLEAGLPKGVLSVVHGDKAAVDALIEAPEVKALSFVGSTPIAEYIYAEGTRRG 240 Query: 241 KRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQIADALVQK 300 KRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQ+ADALV K Sbjct: 241 KRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQVADALVSK 300 Query: 301 LVPQIKGLKIGAGTSCGLDMGPLVTGAARDKVTGYIDTGVAQGAELVVDGRGYKVAGHEN 360 LVPQIK LKIGAGTSCGLDMGPLVTG RDKV+GYI+ GVA GA L+VDGRG V GHE Sbjct: 301 LVPQIKALKIGAGTSCGLDMGPLVTGQHRDKVSGYIEEGVAAGASLIVDGRGLSVTGHEE 360 Query: 361 GFFLGGTLFDRVTPEMTIYKEEIFGPVLCIVRVNSLEEAMQLINDHEYGNGTCIFTRDGE 420 GFFLGG LFDRVTPEM IYKEEIFGPVLCIVRVNSLEEAMQLINDHEYGNGTCIFTRDGE Sbjct: 361 GFFLGGCLFDRVTPEMRIYKEEIFGPVLCIVRVNSLEEAMQLINDHEYGNGTCIFTRDGE 420 Query: 421 AARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTKRKAITQR 480 AARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYT+RKAITQR Sbjct: 421 AARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTRRKAITQR 480 Query: 481 WPQRKSHEAAQFAFPS 496 WPQR SHEA+QFAFPS Sbjct: 481 WPQRASHEASQFAFPS 496 Lambda K H 0.319 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 913 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 498 Length of database: 497 Length adjustment: 34 Effective length of query: 464 Effective length of database: 463 Effective search space: 214832 Effective search space used: 214832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate Pf6N2E2_3462 (Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27))
to HMM TIGR01722 (mmsA: methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01722.hmm # target sequence database: /tmp/gapView.23337.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01722 [M=477] Accession: TIGR01722 Description: MMSDH: methylmalonate-semialdehyde dehydrogenase (acylating) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-212 692.0 0.0 2.2e-212 691.8 0.0 1.0 1 lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 Methylmalonate-semialdehyde dehy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 691.8 0.0 2.2e-212 2.2e-212 3 477 .] 6 481 .. 4 481 .. 0.99 Alignments for each domain: == domain 1 score: 691.8 bits; conditional E-value: 2.2e-212 TIGR01722 3 hlidGkfvegksdkyipvsnpatnevlakvaeasaeevdaavasaretfaawaetsvaerarvl 66 hli+G+++ + s++ +v np t++ + kv+ a + +++a+ +a+ +f+aw++t+ a+ra+v+ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 6 HLINGELL-SDSARTADVFNPSTGQAIHKVPLADRATIQQAIDAAKAAFPAWRNTPAAKRAQVM 68 89999887.679999************************************************* PP TIGR01722 67 lryqallkehrdeiaklisaeqGktledakGdvarGlevvehacsvtslllGetvesvakdvdv 130 +r+++ll++++ ia+lis e+Gktleda G++ rG+e ve+ac+ + +l+Ge + +v ++d lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 69 FRFKQLLEQNESRIAQLISEEHGKTLEDAAGELKRGIENVEFACAAPEILKGEYSRNVGPNIDA 132 **************************************************************** PP TIGR01722 131 ysirqplGvvaGitpfnfpamiplwmfplaiacGntfvlkpsekvpsaavklaellseaGapdG 194 +s qplGvvaGitpfnfpam+plwm+plai+cGn+f+lkpse++ps+++ +a+ll+eaG+p+G lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 133 WSDFQPLGVVAGITPFNFPAMVPLWMYPLAIVCGNCFILKPSERDPSSTLLIAQLLLEAGLPKG 196 **************************************************************** PP TIGR01722 195 vlnvvhGdkeavdrllehpdvkavsfvGsvavgeyiyetgsahgkrvqalaGaknhmvvlpdad 258 vl vvhGdk avd l+e p+vka+sfvGs++++eyiy g+++gkrvqal+Gaknh+v++pdad lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 197 VLSVVHGDKAAVDALIEAPEVKALSFVGSTPIAEYIYAEGTRRGKRVQALGGAKNHAVLMPDAD 260 **************************************************************** PP TIGR01722 259 keaaldalvgaavGaaGqrcmaisaavlvGaa..kelveeireraekvrvgagddpgaelGpli 320 +++a++al+gaa+G+ G+rcmais+av vG+ + lv +++ +++ +++gag+ g ++Gpl+ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 261 LDNAVSALMGAAYGSCGERCMAISVAVCVGDQvaDALVSKLVPQIKALKIGAGTSCGLDMGPLV 324 ******************************855699**************************** PP TIGR01722 321 tkqakervasliasgakeGaevlldGrgykveGyeeGnfvGitllervkpdmkiykeeifGpvl 384 t q++++v+ +i++g++ Ga++++dGrg+ v G+eeG f+G +l++rv p+m+iykeeifGpvl lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 325 TGQHRDKVSGYIEEGVAAGASLIVDGRGLSVTGHEEGFFLGGCLFDRVTPEMRIYKEEIFGPVL 388 **************************************************************** PP TIGR01722 385 vvleadtleeaiklinespyGnGtaiftsdGaaarkfqheievGqvGvnvpipvplpffsftGw 448 +++++++leea++lin+ yGnGt+ift+dG aar f eievG+vGvnvp+pvp++++sf+Gw lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 389 CIVRVNSLEEAMQLINDHEYGNGTCIFTRDGEAARLFCDEIEVGMVGVNVPLPVPVAYHSFGGW 452 **************************************************************** PP TIGR01722 449 kdslfGdlhiyGkqGvrfytrlktvtarw 477 k slfGdlh+yG +Gvrfytr k++t rw lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3462 453 KRSLFGDLHAYGPDGVRFYTRRKAITQRW 481 ***************************** PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (477 nodes) Target sequences: 1 (497 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 9.52 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory