GapMind for catabolism of small carbon sources


Finding step D-LDH for D-lactate catabolism in Desulfovibrio vulgaris Hildenborough

5 candidates for D-LDH: D-lactate dehydrogenase

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other-bits
hi DVU0253 oxidoreductase, FAD/iron-sulfur cluster-binding domain protein Respiratory FAD-dependent D-lactate dehydrogenase Dld; EC (characterized, see rationale) 46% 99% 839
lo DVU0390 glycolate oxidase, subunit GlcD, putative D-lactate dehydrogenase (EC; D-lactate dehydrogenase (acceptor) (EC (characterized) 36% 94% 250.8 lactate dehydrogenase (NAD+, ferredoxin) (subunit 1/3) (EC 33% 267.7
lo DVU0827 glycolate oxidase, subunit GlcD, putative D-lactate dehydrogenase (EC; D-lactate dehydrogenase (acceptor) (EC (characterized) 34% 99% 250.4 D-lactate oxidase, FAD-linked subunit (EC 35% 262.3
lo DVU3027 glycolate oxidase, subunit GlcD D-lactate dehydrogenase (EC (characterized) 32% 95% 246.1 D-lactate oxidase and glycolate oxidase, FAD-linked subunit (EC 39% 341.3
lo DVU1412 D-isomer specific 2-hydroxyacid dehydrogenase family protein D-lactate dehydrogenase (EC (characterized) 40% 81% 176.8 Hydroxypyruvate reductase; EC 49% 332.4

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Definition of step D-LDH

Or cluster all characterized D-LDH proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory