GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gabD in Desulfovibrio vulgaris Hildenborough

Align succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized)
to candidate 208845 DVU3319 proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase

Query= BRENDA::P25526
         (482 letters)



>lcl|MicrobesOnline__882:208845 DVU3319 proline
           dehydrogenase/delta-1-pyrroline-5-carboxylate
           dehydrogenase
          Length = 1006

 Score =  224 bits (571), Expect = 1e-62
 Identities = 149/462 (32%), Positives = 227/462 (49%), Gaps = 13/462 (2%)

Query: 21  DANNGEAIDVTNPAN-GDKLGSVPKMGADETRAAIDAANRALPAWRALTAKERATILRNW 79
           D    + I  TNPA   + + S+ + G  E   AI AA +A   WR  +  +RA  LR  
Sbjct: 520 DVTTADLIPTTNPAKPAEVVASICQAGRPEIDDAIAAAKKAALTWRDTSPADRAAYLRRA 579

Query: 80  FNLMMEHQDDLARLMTLEQGKPLAEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKR 139
            ++  +   +L+    +E GK   +A  +++    F+E++A E  R+      G    + 
Sbjct: 580 ADICRKRIWELSAWQVVEVGKQWDQAYHDVTEGIDFLEYYAREMLRLGAPRRMGRAPGEH 639

Query: 140 LIVIKQPIGVTAAITPWNFPAAMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRA 199
             +  QP G+ A I PWNFP A+    A  A+  G  ++ KP+S +      LAE+   A
Sbjct: 640 NHLFYQPKGIAAVIAPWNFPFAIAIGMASAAIVTGNPVIFKPSSISSRIGYNLAEVFREA 699

Query: 200 GVPAGVFNVVTGSAGAVGNELTSNPLVRKLSFTGSTEIGRQLMEQCAK------DIKKVS 253
           G+P GVFN   G +  +G+ L  +P +  + FTGS E+G ++ E+ AK        K+V 
Sbjct: 700 GLPEGVFNYCPGRSSIMGDYLVEHPDISLICFTGSMEVGLRIQEKAAKVQPGQRQCKRVI 759

Query: 254 LELGGNAPFIVFDDADLDKAVEGALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAV 313
            E+GG    I+ DDADLD+AV   L S F   GQ C   +R+ V D +YDRF E+L +A 
Sbjct: 760 AEMGGKNATIIDDDADLDEAVLQVLYSAFGFQGQKCSACSRVIVLDAIYDRFIERLVKAA 819

Query: 314 SKLHIGDGLDNGVTIGPLIDEKAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTIL 373
           S +HIG   D    +GP+ D      V ++I  A E+G RV+         G +   TI+
Sbjct: 820 SSIHIGPSEDPSNYMGPVADATLQKNVSDYIRIAEEEG-RVLLKRTDLPAEGCYVPLTIV 878

Query: 374 VDVPANAKVSKEETFGPLAPLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEAL 433
            D+    ++++EE FGP+  + R     + ++ AN T F L    ++R    + +     
Sbjct: 879 GDIRPEHRIAQEEIFGPVLAVMRAATFDEALSIANGTRFALTGAVFSRSPEHLDKARREF 938

Query: 434 EYGIVGINTGIISN--EVAPFGGIKASGLGREGSKYGIEDYL 473
             G + +N G      E  PFGG   SG+   GSK G  DYL
Sbjct: 939 RVGNLYLNKGSTGALVERQPFGGFAMSGV---GSKTGGPDYL 977


Lambda     K      H
   0.318    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1062
Number of extensions: 68
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 482
Length of database: 1006
Length adjustment: 39
Effective length of query: 443
Effective length of database: 967
Effective search space:   428381
Effective search space used:   428381
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory