GapMind for catabolism of small carbon sources

 

Alignments for a candidate for peb1C in Desulfovibrio vulgaris Hildenborough

Align PEB1C, component of Uptake system for glutamate and aspartate (characterized)
to candidate 207830 DVU2343 amino acid ABC transporter, ATP-binding protein

Query= TCDB::A3ZI83
         (242 letters)



>MicrobesOnline__882:207830
          Length = 242

 Score =  249 bits (635), Expect = 5e-71
 Identities = 128/241 (53%), Positives = 175/241 (72%), Gaps = 2/241 (0%)

Query: 1   MIELKNVNKYYGTHHVLKNINLSVKEGEKLVIIGPSGSGKSTTIRCMNGLEEVSSGEVVV 60
           MIE  +V+K+YG  HVL+ I   V++GE LVI GPSGSGKS+ IRC+N LE +  G++++
Sbjct: 3   MIEFHDVHKWYGEFHVLRGITQKVEKGEVLVICGPSGSGKSSFIRCLNRLEPIQKGQILL 62

Query: 61  NNLVLNHKN-KIEICRKYCAMVFQHFNLYPHMTVLQNLTLAPMKLQKKSKKEAEETAFKY 119
               ++ K+  +   R    +VFQ FNLYPH++VL+N+TLAP+K++K  + +AE  A + 
Sbjct: 63  EGKSIHDKDVDVNDLRTEVGIVFQQFNLYPHLSVLKNVTLAPIKVRKMPRAKAEAVAMEL 122

Query: 120 LKVVGLLDKANVYPATLSGGQQQRVAIARSLCTKKPYILFDEPTSALDPETIQEVLDVMK 179
           L+ VG+ D+A  YP  LSGGQQQRVAIAR+L  +   +LFDEPTSALDPE I EVL+ MK
Sbjct: 123 LERVGIHDQAGKYPVELSGGQQQRVAIARALAMQPKVMLFDEPTSALDPEMINEVLNCMK 182

Query: 180 EISHQSNTTMVVVTHEMGFAKEVADRIIFMEDGAIVEENIPSEFFSNPKTERARLFLGKI 239
           +++ +   TM+ VTHEMGFA+EVADR+IFM+ G +VEE  P  FFSNP+ ER + FL +I
Sbjct: 183 DLARE-GMTMLCVTHEMGFAREVADRVIFMDGGNVVEEAPPDIFFSNPQHERTKAFLREI 241

Query: 240 L 240
           L
Sbjct: 242 L 242


Lambda     K      H
   0.317    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 167
Number of extensions: 3
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 242
Length of database: 242
Length adjustment: 23
Effective length of query: 219
Effective length of database: 219
Effective search space:    47961
Effective search space used:    47961
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory