Align protein-Npi-phosphohistidine-D-fructose phosphotransferase (subunit 1/2) (EC 2.7.1.202) (characterized)
to candidate 207082 DVU1630 PTS system, IIA component
Query= BRENDA::Q8DWE6 (150 letters) >MicrobesOnline__882:207082 Length = 149 Score = 64.7 bits (156), Expect = 6e-16 Identities = 42/143 (29%), Positives = 76/143 (53%), Gaps = 1/143 (0%) Query: 2 IDENLIKINLICESQDEVFHYLATLVVDNGYANNTESVVQALKLRESEGTTGMMEGFAIP 61 + ++LI + +++EV L V + + ++ L RE GTTG+ +G AIP Sbjct: 7 LSKDLIIHEISASTKEEVLAELVNAVCRAHPEVDGRAALKVLHEREQLGTTGIGDGIAIP 66 Query: 62 HAKDKSIVKPSIAILKLKTGVEWHSMDGQLINNVIALFIPEKEAGTTHLKVLSQIARLLV 121 H K + + +++ + G + S+DG+ ++ V + PE AG HL+VL+ I+RLL Sbjct: 67 HGKLSDLGRIVLSVGRSSAGFGFDSLDGKPVHIVFLVLAPEHVAG-MHLRVLAHISRLLK 125 Query: 122 NKTFKEKIKEADTILELKELLTE 144 + F+ + EA + ELL++ Sbjct: 126 DVAFRRALIEAPDANAMWELLSK 148 Lambda K H 0.315 0.133 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 46 Number of extensions: 4 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 150 Length of database: 149 Length adjustment: 16 Effective length of query: 134 Effective length of database: 133 Effective search space: 17822 Effective search space used: 17822 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 42 (20.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory