GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Desulfovibrio vulgaris Hildenborough

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate 208845 DVU3319 proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase

Query= BRENDA::P25553
         (479 letters)



>lcl|MicrobesOnline__882:208845 DVU3319 proline
           dehydrogenase/delta-1-pyrroline-5-carboxylate
           dehydrogenase
          Length = 1006

 Score =  234 bits (597), Expect = 1e-65
 Identities = 157/465 (33%), Positives = 238/465 (51%), Gaps = 15/465 (3%)

Query: 8   PMYIDGQFVTWRGDAWIDVVNPATEA-VISRIPDGQAEDARKAIDAAERAQPEWEALPAI 66
           P+YI G+ VT      I   NPA  A V++ I      +   AI AA++A   W      
Sbjct: 513 PLYIGGRDVTTAD--LIPTTNPAKPAEVVASICQAGRPEIDDAIAAAKKAALTWRDTSPA 570

Query: 67  ERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGEI 126
           +RA++LR+ +   R+R  E+SA  V E GK    A  +V    D+++Y A    R     
Sbjct: 571 DRAAYLRRAADICRKRIWELSAWQVVEVGKQWDQAYHDVTEGIDFLEYYAREMLRLGAPR 630

Query: 127 IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNAI 186
                PGE+  LF +  G+   I PWNFPF +     + A++TGN ++ KPS  +     
Sbjct: 631 RMGRAPGEHNHLFYQPKGIAAVIAPWNFPFAIAIGMASAAIVTGNPVIFKPSSISSRIGY 690

Query: 187 AFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAK--- 243
             A++  E GLP GVFN   GR   +G  L  +P ++++  TGS+  G +I   AAK   
Sbjct: 691 NLAEVFREAGLPEGVFNYCPGRSSIMGDYLVEHPDISLICFTGSMEVGLRIQEKAAKVQP 750

Query: 244 ---NITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQ 300
                 +V  E+GGK   I+ DDADL+ AV  ++ S     GQ C+   RV V   IYD+
Sbjct: 751 GQRQCKRVIAEMGGKNATIIDDDADLDEAVLQVLYSAFGFQGQKCSACSRVIVLDAIYDR 810

Query: 301 FVNRLGEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEG 360
           F+ RL +A  ++  G P+E     MGP+ +A   + V   +  A EEG RV      +  
Sbjct: 811 FIERLVKAASSIHIG-PSEDPSNYMGPVADATLQKNVSDYIRIAEEEG-RVLLKRTDLPA 868

Query: 361 KGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNL 420
           +G Y P T++ D+R E  I  EE FGPVL V+   T ++A+S+AN + + LT ++++++ 
Sbjct: 869 EGCYVPLTIVGDIRPEHRIAQEEIFGPVLAVMRAATFDEALSIANGTRFALTGAVFSRSP 928

Query: 421 NVAMKAIKGLKFGETYINRENFEAM---QGFHAGWRKSGIGGADG 462
               KA +  + G  Y+N+ +  A+   Q F  G+  SG+G   G
Sbjct: 929 EHLDKARREFRVGNLYLNKGSTGALVERQPF-GGFAMSGVGSKTG 972



 Score = 29.3 bits (64), Expect = 7e-04
 Identities = 23/86 (26%), Positives = 39/86 (45%), Gaps = 6/86 (6%)

Query: 19  RGDAWIDVVNPATEAVISRIP-DGQAEDARKAIDAAERAQPEWEALPA---IERASW--L 72
           R D +   V+   EA +S    +  A+   + +DA  R Q +W+ALP    +E   W   
Sbjct: 137 RKDGFTFTVDLLGEATVSEEESEAYAQGYHEVVDAIAREQEKWKALPGNGPVEGFDWGAT 196

Query: 73  RKISAGIRERASEISALIVEEGGKIQ 98
            K++  I+  A    A  V+  G ++
Sbjct: 197 PKVNVSIKPSALYSQAKPVDVEGSVR 222


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1042
Number of extensions: 49
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 479
Length of database: 1006
Length adjustment: 39
Effective length of query: 440
Effective length of database: 967
Effective search space:   425480
Effective search space used:   425480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory