GapMind for catabolism of small carbon sources

 

Aligments for a candidate for fucO in Desulfovibrio vulgaris Hildenborough

Align Lactaldehyde reductase; Propanediol oxidoreductase; EC 1.1.1.77 (characterized)
to candidate 207887 DVU2396 alcohol dehydrogenase

Query= SwissProt::P0A9S1
         (382 letters)



>lcl|MicrobesOnline__882:207887 DVU2396 alcohol dehydrogenase
          Length = 380

 Score =  228 bits (582), Expect = 2e-64
 Identities = 135/372 (36%), Positives = 204/372 (54%), Gaps = 6/372 (1%)

Query: 13  FGRGAVGALTDEVKRRGYQKALIVTDKTLVQCGVVAKVTDKMDAAGLAWAIYDGVVPNPT 72
           FG G++  L    +R G  + L+V+DK L   G V +V D ++A GL W  ++    NP 
Sbjct: 13  FGNGSLNHLAPCARRLGAARVLLVSDKGLEASGWVERVVDILEADGLEWVYFNDCNSNPR 72

Query: 73  ITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNPEFADVRSLEGLSPTNKPSV 132
              V  G  V+Q   AD +IA+GGGSP DT K IG I  N     +   EG +   +P  
Sbjct: 73  DHQVHHGARVYQEERADVVIALGGGSPMDTAKGIGTIVGNG--GRINDYEGANRIMRPLP 130

Query: 133 PILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFIDADMMDGMPPALKAAT 192
           P++ +PTTAG+ ++++   +ITD E+R K   +    +P V+ ID  ++  M   L  A+
Sbjct: 131 PMIFLPTTAGSGSDISQFCIITDVERRLKMSIISRSLVPNVSIIDPQVLMTMSEELVIAS 190

Query: 193 GVDALTHAIEGYITRGAWALTDALHIKAIEIIAGALRGSV-AGDKDAGEEMALGQYVAGM 251
            +DA  HA+E Y++R A   TD   ++A+E+I   L  +V   D  A E +++    AGM
Sbjct: 191 AIDAFAHAVESYLSRLASPFTDHQALRAMELIMDNLMPAVERRDPAALERLSIASTEAGM 250

Query: 252 GFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTGEKYRDIAR-VMGVKV 310
            FSN GLG+ H +AH LG  ++  HG+ + +LLPHVMR+N   + EK   I R V+G +V
Sbjct: 251 SFSNAGLGIGHSLAHSLGGMFDVMHGLVHPVLLPHVMRFNLPVSVEKLAAIGRIVVGPRV 310

Query: 311 EGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREAT 370
              S E    A +E +      +G+P  L  +   +  +  +A+ AL+D CT  NPREAT
Sbjct: 311 --ASAECIARAGIERLHEFFSALGVPVRLGQLLPDRGVLETIARTALNDACTVTNPREAT 368

Query: 371 LEDIVELYHTAW 382
            E+++ L   AW
Sbjct: 369 CENLLALCEEAW 380


Lambda     K      H
   0.319    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 367
Number of extensions: 19
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 380
Length adjustment: 30
Effective length of query: 352
Effective length of database: 350
Effective search space:   123200
Effective search space used:   123200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory