Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate 206141 DVU0716 branched-chain amino acid ABC transporter, ATP-binding protein
Query= uniprot:Q1MCU3 (247 letters) >MicrobesOnline__882:206141 Length = 238 Score = 240 bits (613), Expect = 2e-68 Identities = 128/235 (54%), Positives = 168/235 (71%), Gaps = 2/235 (0%) Query: 12 LQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVFE 71 L++ + YGN+ AL G+D+ V++GEIV+++GANGAGK+T +M+I G + G V F Sbjct: 3 LELRNLHVKYGNVEALHGIDIRVDEGEIVTILGANGAGKTTTLMSISGLVKPSEGGVFFR 62 Query: 72 GRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKHFAE-DVEKIFTLF 130 + ++ +HE+ I QSPEGRR+F ++VLENL +GA K E + IF LF Sbjct: 63 DEPLHKLHSHEVVARGITQSPEGRRVFGTLSVLENLYLGAFTCRDKARVERTLGWIFELF 122 Query: 131 PRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKLN 190 PRL+ER Q GTLSGGEQQML+IGRALM PK+LLLDEPSLGLAP++VK IF+ +R +N Sbjct: 123 PRLEERRGQLAGTLSGGEQQMLAIGRALMGDPKVLLLDEPSLGLAPILVKSIFDTVRTIN 182 Query: 191 EAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAYLEGG 245 ++ G+TV LVEQNA AAL+L+ R YVM G+V M S LLANPEV+AAYL GG Sbjct: 183 QS-GVTVVLVEQNARAALKLATRGYVMEVGRVVMEDSAASLLANPEVQAAYLGGG 236 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 190 Number of extensions: 6 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 238 Length adjustment: 23 Effective length of query: 224 Effective length of database: 215 Effective search space: 48160 Effective search space used: 48160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory