Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate 209490 DVU0551 high-affinity branched-chain amino acid ABC transporter ATP-binding protein
Query= uniprot:Q1MCU3 (247 letters) >MicrobesOnline__882:209490 Length = 234 Score = 261 bits (667), Expect = 9e-75 Identities = 136/233 (58%), Positives = 174/233 (74%), Gaps = 2/233 (0%) Query: 11 LLQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVF 70 +L++ V TYYGNI+AL +++ + +GEIV+LIGANGAGKST +MTICG+ RTG V+F Sbjct: 1 MLELRNVNTYYGNIQALRDINLTIGEGEIVTLIGANGAGKSTTLMTICGATPPRTGEVLF 60 Query: 71 EGRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKH-FAEDVEKIFTL 129 EG+ I M +EI RL I+Q PEGR IFP +TV ENL +GA L + K A D++ IF L Sbjct: 61 EGKPIHTMKPNEIVRLGISQVPEGRLIFPDLTVQENLDLGAFLRSDKEGIARDLDYIFDL 120 Query: 130 FPRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKL 189 FP L +R Q GGTLSGGEQQML+I RA+M RP+LLLLDEPSLGLAP+I++ IF I+K+ Sbjct: 121 FPILAQRRRQAGGTLSGGEQQMLAISRAIMGRPRLLLLDEPSLGLAPIIIQQIFNIIQKI 180 Query: 190 NEAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAYL 242 N +G TVFLVEQNA AL+++HR YVM G++T+ + LL N EVR AYL Sbjct: 181 N-GDGTTVFLVEQNANQALKIAHRGYVMETGRITLEAPAESLLTNEEVRKAYL 232 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 201 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 234 Length adjustment: 23 Effective length of query: 224 Effective length of database: 211 Effective search space: 47264 Effective search space used: 47264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory