Align anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC 1.1.5.3) (characterized)
to candidate 207410 DVU1940 anaerobic glycerol-3-phosphate dehydrogenase, A subunit, putative
Query= ecocyc::ANGLYC3PDEHYDROGSUBUNITA-MONOMER (542 letters) >MicrobesOnline__882:207410 Length = 516 Score = 376 bits (965), Expect = e-108 Identities = 219/535 (40%), Positives = 291/535 (54%), Gaps = 27/535 (5%) Query: 9 SDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESA 68 + V+IIG GATG G+ARD ALRG+ IL+++ D+ GA+G NHGLLHSG RY TD +A Sbjct: 3 TQVLIIGAGATGTGVARDLALRGVDCILIDQRDVNAGASGGNHGLLHSGGRYVFTDPHAA 62 Query: 69 RECISENQILKRIARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARI 128 EC +E ILKRIA C+E T G F+ + DD ++ + F C +AG+ +D +AR Sbjct: 63 AECKAEGDILKRIAPQCIEETGGYFVAVEGDDPAYASDFPGYCAKAGVPCREVDAHEARR 122 Query: 129 IEPAVNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIREGATVCGVR 188 EPA+ + +V D +VDPF+L+ N+ DA HG L V G E + R Sbjct: 123 NEPAIAESTFAVYEVEDASVDPFKLSLENVADAGRHGGRYLRYTRVLGFDMERGRIVAAR 182 Query: 189 VRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRINQHVINRC 248 V + +G + A V AAG W I A + + AKG+LL+ R+ VINR Sbjct: 183 VEDMRSGREYRIVADEYVVAAGAWSGGITAMAGCHVDIVYAKGTLLVTQTRMAHRVINRL 242 Query: 249 RKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRIL 308 R P D DILVPG T+S++GTTSLR++ DD R T EVD+ + EG + P +A R + Sbjct: 243 RTPGDGDILVPGGTVSVLGTTSLRVE--SPDDVRPTVAEVDVNINEGMGMVPSLADARFI 300 Query: 309 RAYSGVRPLVASDDDPSG--RNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWAT 366 RAY+GVRPLV S PSG R SRG VLLDH E DG+ +TI GGKL TYRLMAE A Sbjct: 301 RAYAGVRPLVRS-GKPSGSDRAASRGFVLLDH-EEDGVHNLVTIAGGKLTTYRLMAERAA 358 Query: 367 DAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEG 426 D VC +LG + PC TA + S E P L+ +R+ DR Sbjct: 359 DLVCERLGVSAPCLTAVRPISPSDEAEWAE-------PGVLQKQNWWRNHDRDD------ 405 Query: 427 RLHRSLVCECEAVTAGEVQYAVENLNVNS----LLDLRRRTRVGMGTCQGELCACRAAGL 482 L+CECE V V + + LL + R+RVG G+CQG C R Sbjct: 406 ----YLLCECEIVPKSGVDSIIATFKDEAEPPRLLAIGLRSRVGKGSCQGAFCGARITAH 461 Query: 483 LQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKE 537 + + + ++ L FL RWKG +PI WG ++E +Y GL G+E E Sbjct: 462 MHDRRIIEGREGLDGLREFLGARWKGQRPILWGAQFNQAELKEALYFGLVGMELE 516 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 769 Number of extensions: 35 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 542 Length of database: 516 Length adjustment: 35 Effective length of query: 507 Effective length of database: 481 Effective search space: 243867 Effective search space used: 243867 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory