Align Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate 209035 DVU0105 glutamine ABC transporter, ATP-binding protein
Query= TCDB::Q9HU32 (257 letters) >MicrobesOnline__882:209035 Length = 244 Score = 269 bits (687), Expect = 5e-77 Identities = 136/249 (54%), Positives = 179/249 (71%), Gaps = 11/249 (4%) Query: 5 TPALEIRNLHKRYGDLEVLKGISLTARDGDVISILGSSGSGKSTFLRCINLLENPHQGQI 64 TP +EIRNLHK YGD VL+GI LT R G+V+ ++G SGSGKST LRCIN LE G I Sbjct: 2 TPMIEIRNLHKSYGDHHVLRGIDLTVRTGEVVVVIGPSGSGKSTALRCINRLEEITSGTI 61 Query: 65 LVSGEELRLKKSKNGDLVAADSQQINRLRSELGFVFQNFNLWPHMSILDNVIEAPRRVLG 124 +V G +L K+ IN +R+E G VFQ FNL+PHMS+L+NV P +V Sbjct: 62 IVDGYDLYDPKT-----------DINHVRTEAGMVFQQFNLFPHMSVLENVTIGPVKVRR 110 Query: 125 KSKAEAIEIAEGLLAKVGIADKRHSYPAQLSGGQQQRAAIARTLAMQPKVILFDEPTSAL 184 ++ EA + LL KVG+ADK H+YP QLSGGQ+QR AIAR+LAMQPKV+LFDEPTSAL Sbjct: 111 MARQEAQALGLALLEKVGLADKAHAYPDQLSGGQKQRVAIARSLAMQPKVLLFDEPTSAL 170 Query: 185 DPEMVQEVLNVIRALAEEGRTMLLVTHEMSFARQVSSEVVFLHQGLVEEQGTPQQVFENP 244 DPE+V EVL V++ LA EG TM++VTHEM FAR+V+ V+F+ G ++E+G P ++F +P Sbjct: 171 DPELVGEVLEVMKQLAREGMTMVVVTHEMGFAREVADRVIFIDYGKIQEEGPPNELFADP 230 Query: 245 QSARCKQFM 253 ++ R ++F+ Sbjct: 231 KNPRLREFL 239 Lambda K H 0.317 0.133 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 176 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 257 Length of database: 244 Length adjustment: 24 Effective length of query: 233 Effective length of database: 220 Effective search space: 51260 Effective search space used: 51260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory