Aligments for a candidate for pccA in Desulfovibrio vulgaris Hildenborough

GapMind for catabolism of small carbon sources

Aligments for a candidate for pccA in Desulfovibrio vulgaris Hildenborough

Align Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (characterized)
to candidate 207299 DVU1834 pyruvate carboxylase, putative

Query= reanno::PS:Dsui_0516
         (663 letters)



>MicrobesOnline__882:207299
          Length = 1234

 Score =  322 bits (824), Expect = 9e-92
 Identities = 181/447 (40%), Positives = 272/447 (60%), Gaps = 9/447 (2%)

Query: 3   KKILIANRGEIACRVIKTAR-KMGIKTVAVYSEADKDALFVEMADEAVCIGPAASKESYL 61
           K IL+ANRG  A R+ ++ R +     V   ++ DK +     A E + +G  A   +YL
Sbjct: 16  KAILVANRGIPARRICRSIRERFDAVAVMTATDVDKTSPAASAAQELLLLG--ADPRAYL 73

Query: 62  VADKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYSIAKMGDKIESKK 121
             D+II+  KQ G  A+HPG+GF SE+  F  +  E G+ FIG    ++  +G+K++ +K
Sbjct: 74  DIDRIISLAKQRGVVAIHPGWGFASEDDRFPSKCHEAGLTFIGSTAEAMNLLGNKVQVRK 133

Query: 122 LAIEAKVNTIPGYNDAIDGPDAAVEIAKKIGYPVMIKASAGGGGKGLRVAYNDAEAHEGF 181
           LA +  V  +PG   A+D P A  ++  +IG P+M+KA  GGGG+G+    +++E  + F
Sbjct: 134 LARKLGVPVVPGSEGAVDIPTAR-KLIDEIGLPIMLKAEGGGGGRGIFAIRDESELDDAF 192

Query: 182 SSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIEE 241
                 A+ SFG+ R+F+EKY+   RHIEIQV+ D +GN    +ERDCSIQR HQK+IE 
Sbjct: 193 FKASTMAQASFGNPRLFVEKYLESVRHIEIQVIADMYGNAFAFDERDCSIQRNHQKLIEI 252

Query: 242 APSPF--VDPEMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHP 299
            PSP+  + PE+R+ + E +  L R V Y S  TVEF+V+ A+ E Y +E+NTRLQVEH 
Sbjct: 253 TPSPWPGITPELREKLKEYSKMLVREVGYHSLATVEFLVT-ASGEAYLIEVNTRLQVEHG 311

Query: 300 VTELITGLDLVEQMIRVAYGEKLPLTQADVQINGWAMECRINAEDPFRGFLPSTGRLVKF 359
           +TE   G+DLVE+ I VA+G +L LT+ + +    AM+ RIN EDP  GF P++G + ++
Sbjct: 312 ITECRYGIDLVEEQIAVAFGAQLRLTEENTKPVHHAMQVRINCEDPQAGFSPNSGLVSRY 371

Query: 360 QPPAEVDGQVRVDTGVYDGGEISMYYDSMIAKLIVHGASREQAIARMRDALNGFVIRGIS 419
             P      VR+D+ +  G E    YDS  + LI +G   ++ +  M   L+ ++I G+ 
Sbjct: 372 VSPG--GPGVRLDSNMCAGYEFPPNYDSAGSLLITYGQGWQKVLGIMERCLSEYIIGGLK 429

Query: 420 SNIPFQAALMQHARFQSGIFDTGFIAK 446
           + IPF   +M+H RF++G FDT FIA+
Sbjct: 430 TTIPFYKQVMKHPRFRAGDFDTNFIAE 456



 Score = 32.0 bits (71), Expect = 2e-04
 Identities = 22/65 (33%), Positives = 32/65 (49%), Gaps = 3/65 (4%)

Query: 585  MPEKAAPDLSKFLLSPMPGLLREVSVAVGQEVKAGEKLAVIEAMKMENILKAEQDCKVKK 644
            M ++A P     + +P  G L  + V  G  VK GE+L  +  MK E  + A  D  VK+
Sbjct: 1120 MADRANP---MHVAAPSNGDLWVMYVHPGDVVKKGEELFNVSIMKQEKAVLAPMDAMVKR 1176

Query: 645  ISVTA 649
            +  TA
Sbjct: 1177 VLKTA 1181


Lambda     K      H
   0.319    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1791
Number of extensions: 93
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 663
Length of database: 1234
Length adjustment: 43
Effective length of query: 620
Effective length of database: 1191
Effective search space:   738420
Effective search space used:   738420
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources