GapMind for catabolism of small carbon sources

 

Aligments for a candidate for paaH in Desulfovibrio vulgaris Hildenborough

Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate 206645 3-oxoacyl-(acyl-carrier-protein) reductase

Query= SwissProt::O18404
         (255 letters)



>lcl|MicrobesOnline__882:206645 3-oxoacyl-(acyl-carrier-protein)
           reductase
          Length = 259

 Score =  114 bits (284), Expect = 3e-30
 Identities = 81/252 (32%), Positives = 126/252 (50%), Gaps = 21/252 (8%)

Query: 7   SLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVA----KELGDKVVFVPVDVTSE 62
           ++VTGG+ G+G+A AE LA+ G  V L  +      E      ++ G       +DV+  
Sbjct: 20  AIVTGGSRGIGKAVAETLARAGLQVFLTYVSKPDEAEAVAAGIRDAGGSATAFRLDVSDA 79

Query: 63  KDVSAALQTA-KDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVGTFN 121
             V+A  Q+  KDK  RLD+ VN AG                  EDF+RV+++N  G F 
Sbjct: 80  AAVAAFFQSEIKDKV-RLDVLVNNAGITKDGLIMRMKD------EDFERVLDVNLCGAFT 132

Query: 122 VIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIARDLS 181
            +R ++ LM         + G I+N  SV    G  GQA Y A+KA ++G+T   A++L+
Sbjct: 133 CLREASKLMTRQ------RLGRIINITSVVGQMGNAGQANYCAAKAGLIGLTKSAAKELA 186

Query: 182 TQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQ--AIYENPL 239
            + + +  +APG   T M A LPE+VR    ++IP  +RLG   + A  V   A      
Sbjct: 187 ARNVTVNAVAPGFIETDMTAGLPEEVRKAYVEAIPL-RRLGSAQDIADAVAFLASERASY 245

Query: 240 LNGEVIRIDGAL 251
           + G+V+ ++G +
Sbjct: 246 ITGQVLAVNGGM 257


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 166
Number of extensions: 9
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 259
Length adjustment: 24
Effective length of query: 231
Effective length of database: 235
Effective search space:    54285
Effective search space used:    54285
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory