Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate 209489 DVU0550 high-affinity branched-chain amino acid ABC transporter, ATP binding protein
Query= uniprot:G8ALJ0 (294 letters) >MicrobesOnline__882:209489 Length = 262 Score = 226 bits (576), Expect = 4e-64 Identities = 129/264 (48%), Positives = 174/264 (65%), Gaps = 10/264 (3%) Query: 11 LLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPTVGRLTL 70 +L V ++ FGGL A+N+V + GEI A+IGPNGAGKTT FNCITG Y PT G + + Sbjct: 4 VLDVRSISKNFGGLRALNEVDLRVDAGEIVALIGPNGAGKTTFFNCITGIYVPTEGEVVV 63 Query: 71 RHADGKEFLLERMPGYRISQ--KASVARTFQNIRLFGGMSVLENLIVAQHNKLIRASGFS 128 + R+ G + +Q + +ARTFQNIRLF M+VLEN+++ H + SG Sbjct: 64 TRPGSEPV---RVNGQKPNQVTELGMARTFQNIRLFQNMTVLENVMIGCHCRT--RSG-- 116 Query: 129 IAGLLGLPSYTR-TEREAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMCTEP 187 I G L + TR E+E +D + L V L + +A NLPYGAQRRLEIARA+ T P Sbjct: 117 ILGALLRDAKTRGEEQEIIDKSYELLKSVHLQQHYKEQARNLPYGAQRRLEIARALATNP 176 Query: 188 VMLCLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDYGRK 247 +L LDEPAAG+NP+E+ EL L+ IR+ + VLLIEHDM++VM++SD + V++YG K Sbjct: 177 FLLLLDEPAAGMNPQETLELKGLVLEIRERLNLSVLLIEHDMNMVMSLSDRIYVMEYGSK 236 Query: 248 ISDGDPAFVKNDPAVIRAYLGEEE 271 I++G P V +P VI+AYLGEEE Sbjct: 237 IAEGTPEEVSRNPRVIKAYLGEEE 260 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 197 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 294 Length of database: 262 Length adjustment: 25 Effective length of query: 269 Effective length of database: 237 Effective search space: 63753 Effective search space used: 63753 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory