Alignments for a candidate for rhaT' in Desulfovibrio vulgaris Hildenborough

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Desulfovibrio vulgaris Hildenborough

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate 206505 DVU1070 branched chain amino acid ABC transporter, ATP-binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>MicrobesOnline__882:206505
          Length = 524

 Score =  249 bits (636), Expect = 2e-70
 Identities = 153/486 (31%), Positives = 255/486 (52%), Gaps = 12/486 (2%)

Query: 16  DAPAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEG 75
           D   ++ + GI + F  V+A  ++++ + PG + AL+GENGAGKSTL+ IL+G    + G
Sbjct: 30  DVTPVVRLEGIGKSFGPVRANHDITLDIVPGRIKALLGENGAGKSTLMSILSGRLAQDTG 89

Query: 76  EILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRTIDWQTMN 135
            I VDG    F S + A+ AG+  ++Q  +L D +TVAEN+ LG +         W +  
Sbjct: 90  IIHVDGEAVRFRSPKDALKAGIGMVYQHFMLVDSMTVAENVLLGQSGA-------WLSPV 142

Query: 136 SRSKA---LLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKE 192
             S+    L      +IDP  R+ DLS+ +R  V I + L  ++R++I+DEPTA L+  E
Sbjct: 143 HMSRVVAELAARYGLDIDPAARVCDLSMGERQRVEILKLLYRDSRVLILDEPTAVLTPGE 202

Query: 193 IDDLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRKTPQDEIVR 252
            + LF  +  + E GKAI+FISHK  E+  +AD+  +  R         S    + E+  
Sbjct: 203 TEQLFEALHRMAENGKAIVFISHKMQEVLALADEIAILRRGEVVDEFHESEVPGEAELAN 262

Query: 253 MMVGRDVENVFPKIDVAIGGPVLEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSEL 312
            MVGR+V        +  G  VL +   +     + +SF +RKGE+  + G+ G G+ EL
Sbjct: 263 RMVGREVILEVAAEPLEPGDRVLHVDGLA-GDGLKGLSFEVRKGEVFAIAGVAGNGQREL 321

Query: 313 SQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLP 372
            + + G+ +P  G++ L G        +   + G+ Y+PE+R      L + +  N  L 
Sbjct: 322 VECVTGLRRPAEGEVELLGIPWRQFFTKAPRQGGLAYIPEDRQGLATCLSLDLVDNFLLT 381

Query: 373 SLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPK 432
           +    +R  FL   +  A AR      +++      P  +LSGGN QK+V+G+     P 
Sbjct: 382 ARGCFTRGPFLDRKSADAAARDILAEYNVQPGRAEAPARSLSGGNLQKLVVGREFYRKPS 441

Query: 433 VIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAG 492
           +I+ + PT+G+DI +   V   + E+ +    +++VS +L E++ ++DRV VM  G   G
Sbjct: 442 LIVAENPTQGLDIAATEEVWARLLEVRSHA-GVLLVSGDLNEVLALADRVAVMYRGCFIG 500

Query: 493 IFERAE 498
           + +R++
Sbjct: 501 LLDRSD 506



 Score = 78.6 bits (192), Expect = 5e-19
 Identities = 66/217 (30%), Positives = 108/217 (49%), Gaps = 11/217 (5%)

Query: 288 DISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGI 347
           DI+  +  G I  + G  GAG+S L   L G     +G + ++G+ +   SP+DA++AGI
Sbjct: 52  DITLDIVPGRIKALLGENGAGKSTLMSILSGRLAQDTGIIHVDGEAVRFRSPKDALKAGI 111

Query: 348 VYVPEERGRHGLALPMPIFQNMTL-PSLARTSRRGFLRAANEFALARKYAERLDLRAAAL 406
             V +      L   M + +N+ L  S A  S     R   E  LA +Y   LD+  AA 
Sbjct: 112 GMVYQH---FMLVDSMTVAENVLLGQSGAWLSPVHMSRVVAE--LAARYG--LDIDPAAR 164

Query: 407 SVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSII 466
              V  LS G +Q+V I K L    +V+ILDEPT  +  G    +   +  +A  G +I+
Sbjct: 165 ---VCDLSMGERQRVEILKLLYRDSRVLILDEPTAVLTPGETEQLFEALHRMAENGKAIV 221

Query: 467 MVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPEA 503
            +S ++ E++ ++D + +++ G     F  +E+  EA
Sbjct: 222 FISHKMQEVLALADEIAILRRGEVVDEFHESEVPGEA 258


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 598
Number of extensions: 38
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 524
Length adjustment: 35
Effective length of query: 477
Effective length of database: 489
Effective search space:   233253
Effective search space used:   233253
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources