Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate 209490 DVU0551 high-affinity branched-chain amino acid ABC transporter ATP-binding protein
Query= TCDB::P21630 (233 letters) >MicrobesOnline__882:209490 Length = 234 Score = 261 bits (666), Expect = 1e-74 Identities = 133/233 (57%), Positives = 172/233 (73%), Gaps = 1/233 (0%) Query: 1 MLSFDKVSTYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRY 60 ML V+TYYG IQAL D+++ + +GEIVTLIGANGAGKST LMT+CG+ +G + + Sbjct: 1 MLELRNVNTYYGNIQALRDINLTIGEGEIVTLIGANGAGKSTTLMTICGATPPRTGEVLF 60 Query: 61 EGEELVGLPSSTIMRKSIAVVPEGRRVFSRLTVEENLAMGGFF-TDKDDYQVQMDKVLEL 119 EG+ + + + I+R I+ VPEGR +F LTV+ENL +G F +DK+ +D + +L Sbjct: 61 EGKPIHTMKPNEIVRLGISQVPEGRLIFPDLTVQENLDLGAFLRSDKEGIARDLDYIFDL 120 Query: 120 FPRLKERYEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFEIIEQL 179 FP L +R Q GT+SGGEQQMLAI RA+M +P+LLLLDEPSLGLAPIIIQQIF II+++ Sbjct: 121 FPILAQRRRQAGGTLSGGEQQMLAISRAIMGRPRLLLLDEPSLGLAPIIIQQIFNIIQKI 180 Query: 180 RREGVTVFLVEQNANQALKLADRAYVLENGRIVMHDTGAALLTNPKVRDAYLG 232 +G TVFLVEQNANQALK+A R YV+E GRI + +LLTN +VR AYLG Sbjct: 181 NGDGTTVFLVEQNANQALKIAHRGYVMETGRITLEAPAESLLTNEEVRKAYLG 233 Lambda K H 0.318 0.136 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 181 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 234 Length adjustment: 23 Effective length of query: 210 Effective length of database: 211 Effective search space: 44310 Effective search space used: 44310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory