Alignments for a candidate for L-LDH in Desulfovibrio vulgaris Hildenborough

GapMind for catabolism of small carbon sources

Alignments for a candidate for L-LDH in Desulfovibrio vulgaris Hildenborough

Align L-lactate dehydrogenase (EC 1.1.1.27) (characterized)
to candidate 209544 DVU0600 L-lactate dehydrogenase

Query= BRENDA::B1A4R6
         (311 letters)



>MicrobesOnline__882:209544
          Length = 309

 Score =  286 bits (733), Expect = 3e-82
 Identities = 139/302 (46%), Positives = 210/302 (69%)

Query: 1   MSKISVIGSGFVGATTAYTLALSGIAKTIVLIDINKDKAEGDALDISHGVPFISPVEVYA 60
           M++I+VIG G VG   AY  A+  +A  IVLID N  +AEG+++D++  +  + PV++ +
Sbjct: 1   MNRIAVIGVGNVGMAFAYAAAIKRLANDIVLIDANAARAEGESMDLADAMALVGPVQIRS 60

Query: 61  GDYGDTVGSDIIIITAGAAQKPGETRLDLVKKNTMIFKDIVAKLIKVNDTAIYLIVTNPV 120
           G Y    G+ I+++TAGA Q PG++RLDLV+ N  I +DI+  +++  D  +Y++ TNPV
Sbjct: 61  GGYEQCEGARIVVVTAGAKQMPGQSRLDLVRVNAGITRDILTAVMQYADDPLYIMATNPV 120

Query: 121 DILTYVTYKISGLPYGRVLGSGTVLDSARFRYLLSKHCNIDPRNIHGYIIGEHGDSELAA 180
           D+LT+V   ++G+  GRV+GSGTVLDSARFR  +++   +D R +H +I+GEHGDSE+A 
Sbjct: 121 DVLTHVARTVTGVAPGRVIGSGTVLDSARFRGHVAEILGVDVRGVHAHIVGEHGDSEVAL 180

Query: 181 WSITNIAGIPIDNYCNLCGKVCEKDFREEIFNNVVRAAYTIIEKKGATYYAVALAVRRIV 240
           WS  N++GIP+   C   G   +  FRE+   +V  AAY II +KGAT Y + +++ RIV
Sbjct: 181 WSRANVSGIPVAEMCARRGIAYDAAFREKALGHVRHAAYEIIGRKGATGYGIGMSLCRIV 240

Query: 241 EAILRDENSILTVSSPLTGQYGVTDVALSLPSVVGRNGIVNILELPLSQEEIAAFRRSAE 300
           EAIL DE+S+LTVS P+ G YG+ DV+LSLP V+G +GI  +L+ P++++E AA   SA 
Sbjct: 241 EAILHDEHSVLTVSCPVAGHYGLGDVSLSLPCVIGSDGIEEVLDAPIAEDEQAALAASAR 300

Query: 301 II 302
           ++
Sbjct: 301 VL 302


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 280
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 311
Length of database: 309
Length adjustment: 27
Effective length of query: 284
Effective length of database: 282
Effective search space:    80088
Effective search space used:    80088
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate 209544 DVU0600 (L-lactate dehydrogenase)
to HMM TIGR01771 (L-lactate dehydrogenase (EC 1.1.1.27))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01771.hmm
# target sequence database:        /tmp/gapView.6565.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01771  [M=299]
Accession:   TIGR01771
Description: L-LDH-NAD: L-lactate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                       -----------
   7.8e-109  349.6   0.3   8.9e-109  349.4   0.3    1.0  1  lcl|MicrobesOnline__882:209544  DVU0600 L-lactate dehydrogenase


Domain annotation for each sequence (and alignments):
>> lcl|MicrobesOnline__882:209544  DVU0600 L-lactate dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  349.4   0.3  8.9e-109  8.9e-109       1     297 [.       6     302 ..       6     304 .. 0.99

  Alignments for each domain:
  == domain 1  score: 349.4 bits;  conditional E-value: 8.9e-109
                       TIGR01771   1 iiGagaVGssvafalltqgladelvliDinedkaegealdlsdaasflkskvkvkagdyedlkdadlvvitaGaeqkpg 79 
                                     +iG+g+VG+++a+a++ + la+++vliD n  +aege +dl+da++++  +v++++g ye++++a++vv+taGa+q pg
  lcl|MicrobesOnline__882:209544   6 VIGVGNVGMAFAYAAAIKRLANDIVLIDANAARAEGESMDLADAMALV-GPVQIRSGGYEQCEGARIVVVTAGAKQMPG 83 
                                     8**********************************************9.88**************************** PP

                       TIGR01771  80 etRlelveknvkilksivkevvksgfdgillvvsNPvDiltyvvlklsglpkerviGsGtvLdtaRlrrllaeklevdp 158
                                     ++Rl+lv+ n+ i ++i++ v++++ d ++++++NPvD+lt+v+++++g+ + rviGsGtvLd+aR+r ++ae l+vd 
  lcl|MicrobesOnline__882:209544  84 QSRLDLVRVNAGITRDILTAVMQYADDPLYIMATNPVDVLTHVARTVTGVAPGRVIGSGTVLDSARFRGHVAEILGVDV 162
                                     ******************************************************************************* PP

                       TIGR01771 159 ksvhayvlGEHGdsevavwssakiagvplkelleekekekekelk.eiekevrdaayeiiekKgaTsygiglavarive 236
                                     + vha+++GEHGdseva+ws+a++ g+p+ e++  ++ + ++  + +   +vr+aayeii +KgaT ygig++++rive
  lcl|MicrobesOnline__882:209544 163 RGVHAHIVGEHGDSEVALWSRANVSGIPVAEMCARRGIAYDAAFReKALGHVRHAAYEIIGRKGATGYGIGMSLCRIVE 241
                                     ***************************************99998879999***************************** PP

                       TIGR01771 237 ailkdekrvlpvsallegeygikdvylgvPavlgknGveeilelkLseeEkeklkksaetl 297
                                     ail+de++vl+vs+ + g+yg  dv l++P+v+g++G+ee+l++++ e+E+++l+ sa++l
  lcl|MicrobesOnline__882:209544 242 AILHDEHSVLTVSCPVAGHYGLGDVSLSLPCVIGSDGIEEVLDAPIAEDEQAALAASARVL 302
                                     **********************************************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (299 nodes)
Target sequences:                          1  (309 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.52
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources