Align D-trehalose PTS system, I, HPr, and IIA components (characterized)
to candidate 206413 DVU0981 multiphosphoryl transfer protein, putative
Query= reanno::WCS417:GFF4500 (838 letters) >MicrobesOnline__882:206413 Length = 854 Score = 415 bits (1067), Expect = e-120 Identities = 275/705 (39%), Positives = 373/705 (52%), Gaps = 44/705 (6%) Query: 152 PSGAVEAVDEEEGDALFSKPLTLPNANGLHARPAAVFAQAAKGFNASIYLHKQTQSANAK 211 PS A+ A +E L +PN GLHARPAA A F A + L + + +A+ Sbjct: 158 PSSAMPAGEE--------LTLVVPNRLGLHARPAARIVTALGPFAADVQLVRGDRVVSAR 209 Query: 212 SLVAIMALQTVQGDTLQVSAAGEDAEAAIKALVALLAEGCGEAVV--------------- 256 S+ I L G+T+ A G DA A++A+ AL A G+A Sbjct: 210 SVNRIATLAVRGGETVTFRAVGGDAALALRAIEALAAAHFGDAPEAPSKGEAPSPAEAPK 269 Query: 257 --NVAEPV--ATQSSATLLRGVCASPGSAFGQVVQVTD----PELVITEQGTGGATERAA 308 ++AE A +LRG ASPG G V P+ + ATE Sbjct: 270 DGDMAEAAVSADVQGGGVLRGAAASPGLTVGNAVWYRPAFDAPD--VAPLADDPATEVTR 327 Query: 309 LTRGLLAANEALQVLQDK---AAGSAQAEIFRAHQELLEDPTLLEHAH-RLLGEGKSAAF 364 L L AA L L+ + AAG +AEIF H+ LL+D T+ A R++ ++A Sbjct: 328 LDAALGAARTELVELERRTVAAAGRKEAEIFAMHRLLLDDVTIAGAARQRIMDRREAAES 387 Query: 365 AWNSATLATVTLFQGLGNALIAERAADLADVGQRVLKLILGIQDSAWDLPERAILIAEQL 424 AW F+ L + ER AD+ DVG RVL+L+ G+ L ++L+A L Sbjct: 388 AWYEVISDAAATFRQLPEGYMREREADMVDVGARVLRLLTGVAAVGPRLGGPSVLLATDL 447 Query: 425 TPSQTASLDTRKVLGFVTVAGGATSHVAILARALGLPAICGVPAQVLALANGKQVLLDAD 484 PS A+LD V+G VTV GGATSH AILAR+LG+PA+ G+ + + G V LD Sbjct: 448 GPSDMATLDPSLVIGIVTVQGGATSHAAILARSLGIPAVAGLGPALQGVGEGDIVALDGG 507 Query: 485 KGELHLEPNLAEIEQLEAARKHQVLRHQRDVAQASLPATTRDGHHVEVTANVASLQEVEH 544 G++ + P +EA R + + +A A+ PA T DG V + AN+ S + Sbjct: 508 TGDVWVNPAPGVRAAVEARRDAWLAGREAALAGAAAPAVTADGRAVHILANIGSSADAGA 567 Query: 545 ALTLGGEGVGLLRSEFLYLDRNRAPSPEEQAGTYTAIARALGTERNLVVRTLDVGGDKPL 604 AL G EGVGL R+EFL+LDR P EEQ Y A A+ R +VVRTLD+GGDKP+ Sbjct: 568 ALKNGAEGVGLFRTEFLFLDRTSPPGEEEQLTAYVTAAAAM-QGRPVVVRTLDIGGDKPV 626 Query: 605 AYVP--MDAETNPFLGLRGIRLCLERPQLLREQFRAILASAGFARLHIMLPMVSLLSELH 662 +Y+ E NPFLGLRGIR CLER L Q RA+L +A L +M PMV+ EL Sbjct: 627 SYLEGFATGEDNPFLGLRGIRFCLERKPLFMTQLRALLRAAAVHPLKVMFPMVAHPGELA 686 Query: 663 LARKILEEEALALGLTELP----KLGIMIEVPSAALMADVFAPHVDFFSIGTNDLTQYTL 718 A+ +L+E L +P +GIMIEVP+A +AD A FFSIGTNDL QY + Sbjct: 687 AAKALLDEARATLDAEGMPHGQLDVGIMIEVPAAVALADQLARDAAFFSIGTNDLAQYVM 746 Query: 719 AMDRDHPRLANQADSFHPAVLRLIATTVKAAHAHGKWVGVCGALASEALAVPVLIGLGVD 778 A DR + +A +D+ HPAVLR++ TV+A HA G V +CG L A+P+L+GL +D Sbjct: 747 AADRGNASVAALSDALHPAVLRMVRDTVRAGHAAGIPVAICGELGGNPEAIPLLVGLELD 806 Query: 779 ELSVSVPLIPTIKATVRELDLADCQIIARQVLGLEEAAEVREALR 823 ELS++ P IP K VR D C ++A + + L +AA VR L+ Sbjct: 807 ELSMNGPAIPRAKEVVRGCDTGTCAVLADRAMALPDAAAVRRLLQ 851 Lambda K H 0.318 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1603 Number of extensions: 84 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 838 Length of database: 854 Length adjustment: 42 Effective length of query: 796 Effective length of database: 812 Effective search space: 646352 Effective search space used: 646352 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory