GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Ac3H11_1694 in Desulfovibrio vulgaris Hildenborough

Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate 206139 DVU0714 branched-chain amino acid ABC transporter, permease protein

Query= uniprot:A0A165KER0
         (358 letters)



>lcl|MicrobesOnline__882:206139 DVU0714 branched-chain amino acid
           ABC transporter, permease protein
          Length = 317

 Score =  210 bits (534), Expect = 5e-59
 Identities = 132/346 (38%), Positives = 193/346 (55%), Gaps = 47/346 (13%)

Query: 1   MKNTKTNWIIGAVALLVLPLILQSFGNAWVRIADLALLYVLLALGLNIVVGYAGLLDLGY 60
           M + K ++   AV + VLPL L  +   W  +     LY +LAL LN+++G AGL  +G+
Sbjct: 1   MISRKASYAGLAVLIAVLPLFLDPY---WTDVCVSIGLYAVLALSLNLILGQAGLFHMGH 57

Query: 61  VAFYAVGAYLFALMASPHLADNFAAFAAMFPNGLHTSLWIVIPVAALLAAFFGAMLGAPT 120
            AFYAVGAY  A++ + +                H  +   +PVA LLAA F  ++  P 
Sbjct: 58  AAFYAVGAYTAAILNTVY----------------HVPVLWTMPVAGLLAALFALVVARPI 101

Query: 121 LKLRGDYLAIVTLGFGEIIRIFLNNLDHPVNLTNGPKGLGQIDSVKVFGLDLGKRLEVFG 180
           + LRGDYL IVT+G  EI+RI L N  +   +T G  G        +FG+    R  +FG
Sbjct: 102 IHLRGDYLLIVTIGIVEIVRIALIN--NVFGITGGANG--------IFGIS---RPMLFG 148

Query: 181 FDINSVTLYYYLFLVLVVVSVIICYRLQDSRIGRAWMAIREDEIAAKAMGINTRNMKLLA 240
           + I+    +YYL    V +S+++  RL+ SR GRA   I+ED++AA+  G++T   KL A
Sbjct: 149 YKISKPIQFYYLIWTWVAISILLFRRLECSRFGRALNYIKEDDVAAEGSGVDTAYYKLAA 208

Query: 241 FGMGASFGGVSGAMFGAFQGFVSPESFSLMESVMIVAMVVLGGIGHIPGVILGAVLLSAL 300
           F +GA + G++G  + A    +SPESFS  ESV++ A+V+LGG G   GV+LGA LL  L
Sbjct: 209 FVLGALWAGMTGTFYAAKMTIISPESFSFWESVVLFAIVILGG-GSNRGVLLGAFLLIGL 267

Query: 301 PEVLRYVAGPLQAMTDGRLDSAILRQLLIALAMIIIMLLRPRGLWP 346
           PE+ R              D A  R L+  LAM+++M+ RP+G+ P
Sbjct: 268 PELFR--------------DFASARMLIFGLAMVVMMIFRPQGMLP 299


Lambda     K      H
   0.328    0.144    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 294
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 358
Length of database: 317
Length adjustment: 28
Effective length of query: 330
Effective length of database: 289
Effective search space:    95370
Effective search space used:    95370
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory