Align Malonate-semialdehyde dehydrogenase 1; MSA dehydrogenase 1; EC 1.2.1.-; Methylmalonate-semialdehyde dehydrogenase 1; MMSA dehydrogenase 1; MSDH 1; EC 1.2.1.27 (uncharacterized)
to candidate 208845 DVU3319 proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase
Query= curated2:Q5L025 (488 letters) >MicrobesOnline__882:208845 Length = 1006 Score = 232 bits (591), Expect = 5e-65 Identities = 160/460 (34%), Positives = 237/460 (51%), Gaps = 16/460 (3%) Query: 14 YIGGQWVASSGTETLEVPNPAT-GEVLARVPISTKEDVDQAVQAAKKAFATWKDVPVPKR 72 YIGG+ V ++ + + NPA EV+A + + + ++D A+ AAKKA TW+D R Sbjct: 515 YIGGRDVTTA--DLIPTTNPAKPAEVVASICQAGRPEIDDAIAAAKKAALTWRDTSPADR 572 Query: 73 ARIMFSFHHLLNQHHEELAELVVQENGKAYKEAYGEIQRGIECVEFAAGAPTLLMGESLS 132 A + + + EL+ V E GK + +AY ++ GI+ +E+ A L Sbjct: 573 AAYLRRAADICRKRIWELSAWQVVEVGKQWDQAYHDVTEGIDFLEYYAREMLRLGAPRRM 632 Query: 133 NIAEEIDSEMFRYPLGVVAGITPFNFPMMVPLWMFPLAIVCGNTFVLKPSERTPILANKL 192 A + +F P G+ A I P+NFP + + M AIV GN + KPS + + L Sbjct: 633 GRAPGEHNHLFYQPKGIAAVIAPWNFPFAIAIGMASAAIVTGNPVIFKPSSISSRIGYNL 692 Query: 193 AELFTEAGAPPGVLNVVHGAHEVV-NALIDHEDIRAISFVGSQPVAKYVYERTA------ 245 AE+F EAG P GV N G ++ + L++H DI I F GS V + E+ A Sbjct: 693 AEVFREAGLPEGVFNYCPGRSSIMGDYLVEHPDISLICFTGSMEVGLRIQEKAAKVQPGQ 752 Query: 246 AQGKRVQALSGAKNHHIVMPDADVETAVQHVISSAFGSAGQRCMACSAVVIVGE-NETFV 304 Q KRV A G KN I+ DAD++ AV V+ SAFG GQ+C ACS V+++ + F+ Sbjct: 753 RQCKRVIAEMGGKNATIIDDDADLDEAVLQVLYSAFGFQGQKCSACSRVIVLDAIYDRFI 812 Query: 305 RRLKQKADELIIGNGMDPEVLLTPVIRQSHREKVLGYIQKGIEEGAVLLRDGRKEMDDRP 364 RL + A + IG DP + PV + ++ V YI+ EEG VLL K D Sbjct: 813 ERLVKAASSIHIGPSEDPSNYMGPVADATLQKNVSDYIRIAEEEGRVLL----KRTDLPA 868 Query: 365 EGNFLGPTIFDYVTPDMTIAKEEIFAPVLSLLRANDLDEALSYIRKSRYGNGATIYTKDA 424 EG ++ TI + P+ IA+EEIF PVL+++RA DEALS +R+ ++++ Sbjct: 869 EGCYVPLTIVGDIRPEHRIAQEEIFGPVLAVMRAATFDEALSIANGTRFALTGAVFSRSP 928 Query: 425 KAVRKFREEADAGMLGINVG-VPATMAFFPFSGWKDSFYG 463 + + K R E G L +N G A + PF G+ S G Sbjct: 929 EHLDKARREFRVGNLYLNKGSTGALVERQPFGGFAMSGVG 968 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1009 Number of extensions: 37 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 488 Length of database: 1006 Length adjustment: 39 Effective length of query: 449 Effective length of database: 967 Effective search space: 434183 Effective search space used: 434183 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory