Alignments for a candidate for actP in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Magnetospirillum magneticum AMB-1

Align Cation/acetate symporter ActP; Acetate permease; Acetate transporter ActP (characterized)
to candidate WP_011386681.1 AMB_RS21920 cation acetate symporter

Query= SwissProt::P32705
         (549 letters)



>NCBI__GCF_000009985.1:WP_011386681.1
          Length = 554

 Score =  668 bits (1723), Expect = 0.0
 Identities = 346/546 (63%), Positives = 418/546 (76%), Gaps = 4/546 (0%)

Query: 5   LTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVVFTLGITYWASKRVRSRSDYY 64
           L  LA +LP  A  AD   G   RQ TNW AI+MF+ FV+ TLGIT+WASKR +S +D+Y
Sbjct: 12  LMLLAPSLPALAAGADL--GQAARQATNWHAILMFVGFVLLTLGITWWASKRTKSAADFY 69

Query: 65  TAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLIYSLGFLVGWPIILFLIAER 124
           TAGG ITGFQNGLAIAGD+MSAASFLG++AL++ +G DG++Y++G +VGWP++LFLIAE 
Sbjct: 70  TAGGGITGFQNGLAIAGDFMSAASFLGVTALLYGTGLDGMVYAVGVVVGWPLMLFLIAEP 129

Query: 125 LRNLGRYTFADVASYRLKQGPIRILSACGSLVVVALYLIAQMVGAGKLIELLFGLNYHIA 184
           LRNLG+YTFADV +YRL + P+R  +A  SL VV  YLIAQMVGAG+LI+LLFG++Y  A
Sbjct: 130 LRNLGKYTFADVVAYRLAKVPVRTYAAFSSLTVVVFYLIAQMVGAGQLIKLLFGMDYLYA 189

Query: 185 VVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFGASFMAFMVMKHVGFSFNNLFSEAMAV 244
           + LVG LMM+YV FGGM ATTWVQIIKAVLLL GA+FMA  V+   GFS   +F++A+ V
Sbjct: 190 LFLVGGLMMIYVTFGGMAATTWVQIIKAVLLLSGATFMAGAVLSRFGFSPEAMFAKAVQV 249

Query: 245 HPKGVDIMKPGGLVKDPISALSLGLGLMFGTAGLPHILMRFFTVSDAREARKSVFYATGF 304
                 IM PG L KDPI  +SL + L FGTAGLPHILMRFFTV DA+EARKSVFYAT F
Sbjct: 250 KGSNA-IMSPGLLFKDPIDTISLAMALAFGTAGLPHILMRFFTVPDAKEARKSVFYATAF 308

Query: 305 MGYFYILTFIIGFGAIMLVGANPEYKDAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVA 364
           +G+FY+L  +IG GAI LV  +P Y      L GG NMAAV LA+AVGG+LFLGFISAVA
Sbjct: 309 IGFFYVLAVVIGIGAIALVATDPTYLADGKALKGGGNMAAVWLAHAVGGDLFLGFISAVA 368

Query: 365 FATILAVVAGLTLAGASAVSHDLYANVFKKG-ATEREELRVSKITVLILGVIAIILGVLF 423
           FATILAVVAGLTLAGASA+SHDLYANVF +G A E  ELR+S++  L LGVIAIILG+LF
Sbjct: 369 FATILAVVAGLTLAGASAISHDLYANVFARGHAKEETELRISRLASLALGVIAIILGMLF 428

Query: 424 ENQNIAFMVGLAFAIAASCNFPIILLSMYWSKLTTRGAMMGGWLGLITAVVLMILGPTIW 483
           E QNIA++ GL  AIAAS NFP++LLSM W  LT+RGA++GG  GL++AVVL +LGP +W
Sbjct: 429 EKQNIAYLAGLTLAIAASANFPLLLLSMLWPGLTSRGAILGGVAGLVSAVVLTVLGPAVW 488

Query: 484 VQILGHEKAIFPYEYPALFSITVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGV 543
             +LG+ + IFP+  PALFS+   F   WFFS TD S    RE+  F AQF+RSQTG G 
Sbjct: 489 KAVLGNPQPIFPWGNPALFSVAAGFAATWFFSVTDGSERAVREKAEFNAQFVRSQTGLGA 548

Query: 544 EQGRAH 549
           E   AH
Sbjct: 549 EGVSAH 554


Lambda     K      H
   0.329    0.142    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 950
Number of extensions: 46
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 554
Length adjustment: 36
Effective length of query: 513
Effective length of database: 518
Effective search space:   265734
Effective search space used:   265734
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources