Align arginine decarboxylase (EC 4.1.1.19) (characterized)
to candidate WP_011383521.1 AMB_RS05630 amino acid decarboxylase
Query= BRENDA::E8X9U7 (756 letters) >NCBI__GCF_000009985.1:WP_011383521.1 Length = 783 Score = 436 bits (1120), Expect = e-126 Identities = 241/748 (32%), Positives = 404/748 (54%), Gaps = 24/748 (3%) Query: 15 DTWVGNAVERLADALSQQNVTVIKSTSFDDGYAILSANEAIDCLMFSYQMEQPDEHLSVR 74 D G V+++ + + V+++ +D + + AI C++ + E+ + Sbjct: 19 DDLEGVRVQQIITEVERLGFEVVRARRIEDAEIAVQTDAAIGCVLVDW--EKGGKGAEAT 76 Query: 75 QLIGKLHERQQNVPVFLLGDREKATASLDRDLLELVDEFAWILEDTADFIAGRAVAAMTR 134 +LI + R +P+ +L E+ + D+L+ +D + ++ E+T +FIA V+ + + Sbjct: 77 ELINLMRRRGLEMPIVILVRGERFE-DIPVDVLDYIDGYVFLAEETPEFIAKNLVSRLKQ 135 Query: 135 YRQQLLPPLFNALMKYSDIHEYSWAAPGHQGGVGFTKTPAGRFYHDYYGENLFRSDMGIE 194 + + L P F AL+ Y++ W PGH GG+ + ++P GR + ++ GE +FR D+ Sbjct: 136 FAETLKTPFFGALVDYAEEGNQLWTCPGHNGGIFYNRSPIGRIFVEHLGEAVFRDDLDNS 195 Query: 195 RTTLGSLLDHTGAFGESEKNAARVFGADRSWSVVVGTSGSNRTIMQACMTDNDVVVLDRN 254 LG LL H G +++K AA++FGA++++ V+ GTS SN+ ++ A + ++D+V+ DRN Sbjct: 196 VLELGDLLTHEGPALQAQKEAAKIFGAEKTYFVLNGTSASNKIVLSALVAEDDLVLFDRN 255 Query: 255 CHKSIEQG-LILTGAKPVYMVPSRNRYGIIGPIYPQEMQPETLQKKISASPLTK-TKAGQ 312 HK+ G L+L G P+++ RN G+IGPIY + + ++ KI +PL K +A + Sbjct: 256 NHKAAHHGALLLGGGIPIFLETDRNAMGMIGPIYAEAFDEQAIRAKIRDNPLVKDPEAWK 315 Query: 313 KP---SYSVVTNCTYDGVCYNAKEAQDLLAKTSDRIHFDEAWYGYARFNPIYCDHYAM-R 368 KP +V+ CTYDG Y+A+ + + D I FDEAW G+ +F+P++ Y M Sbjct: 316 KPRPFRVAVIEQCTYDGTIYSAEHILERIGHLCDYILFDEAWAGFLKFHPLFRGCYGMGL 375 Query: 369 GEPGDHNGPTVFATHSTHKLLNALSQASYIHVREGR-----GAVNFSRFNQAYMMHATTS 423 + D + P + +T STHK + SQAS IHVR+ + RFN+ +++HA+TS Sbjct: 376 KDRLDEDSPGIISTQSTHKQMAGFSQASQIHVRDRHIKGQSRRIEHRRFNETFLLHASTS 435 Query: 424 PLYAICASNDVAVSMMDGNSGLSLTQEVIDEAVDFRQAMARLYKEFTDE-----GDWFFK 478 P Y + AS DV MM G SG L + ID ++ R+ + + +EF ++ WFF+ Sbjct: 436 PFYPLFASLDVGAQMMRGRSGEVLWDDTIDLGIELRKKVRAIKREFVEKEKDSARHWFFE 495 Query: 479 PWNKDVVTDPQTGKT-YDFADAPAKLLATDQNCWVMRPGETWHGFKDLP-DNWSMLDPIK 536 P+ D V P+ G+T + D P + +A D W PG WHGFK + ++M DP K Sbjct: 496 PFVPDRVPSPEGGETMVAWEDLPTETIAADPRYWEFTPGARWHGFKHVSRSGYAMTDPNK 555 Query: 537 VSILAPGMG-DDGELEASGVPAALVTAWLGRHGIVPTRTTDFQIMFLFSMGVTRGKWGTL 595 ++++ PG D G G+PA +V +L + +VP + ++FL + GV K GTL Sbjct: 556 LTLITPGFNRDTGAYAEYGIPAPVVAQYLRENRVVPEKNDLNSMLFLLTPGVESSKAGTL 615 Query: 596 INTLCSFKHHYDANTPLAQVMPELVQDYPDTYANMGIHDLGDKMFAWLRENNPGARLNAA 655 +++L +FK +D N L +PE V+ P Y + I DL +M A+ R+ N A Sbjct: 616 LSSLVAFKRRHDDNALLDDAIPEFVRRRPQRYRGLRIQDLCAEMHAFFRDRNVSTLQRAQ 675 Query: 656 YST--LPVAEITPRDAYNAIVNNNIEMVAIENLPGRIAANSVIPYPPGIPMLLSGENFGD 713 +S P + P DA + N ++ + I+ + GRIA + YPPGI ++ GE + Sbjct: 676 FSPEHRPHMVMRPNDAVRMLTRNQVDYLPIDQIDGRIATTLWVVYPPGIATIIPGERLCE 735 Query: 714 ENSPQVGYLRSLQSWDHHFPGFEHETEG 741 P + YL+ + + FPGFE E +G Sbjct: 736 RARPMLDYLKMFERSANLFPGFEAEIQG 763 Lambda K H 0.319 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1410 Number of extensions: 71 Number of successful extensions: 10 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 756 Length of database: 783 Length adjustment: 41 Effective length of query: 715 Effective length of database: 742 Effective search space: 530530 Effective search space used: 530530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory