Alignments for a candidate for drdehyd-beta in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Magnetospirillum magneticum AMB-1

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate WP_011386545.1 AMB_RS21230 xanthine dehydrogenase family protein molybdopterin-binding subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>NCBI__GCF_000009985.1:WP_011386545.1
          Length = 713

 Score =  383 bits (983), Expect = e-110
 Identities = 275/735 (37%), Positives = 372/735 (50%), Gaps = 50/735 (6%)

Query: 14  LSRRRFLASTAVGALVIGFGLPLGAGRVQAATSAERGTQVPAFLEIRPDGTVRLLSPFME 73
           + RR FL +TA      G GL L  G   A T AE G +  AF+ I PDG V +    +E
Sbjct: 10  IDRRTFLITTA------GAGLALAVGDAHADTEAE-GWRPNAFVAIGPDGKVTVTVKHLE 62

Query: 74  GGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVMENGL-----RITGGSMSVRMSY 128
            GQG  T +  +V EEL+ DPA   V  AP   A     N L     + TGGS +   S+
Sbjct: 63  MGQGVATGLPVLVAEELEVDPAQVSVAFAP---ADAKRYNNLFWGPSQGTGGSTATANSW 119

Query: 129 PTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGYGELASSALDMPVP 188
             +R  GA AR ML++A A+        L+   G VVH+ SGR LGYGEL S A  +   
Sbjct: 120 EQLRLAGAAAREMLVEAAARILRADAASLSAVNGGVVHSPSGRRLGYGELVSVAAKLT-- 177

Query: 189 DPASITLRDPSQFRWIGKP-VKRLDAYDKSTGKALYSIDLKVDNMLHAAVQHAPRLGMTV 247
            P++  L+  SQFR IG+  VKR D+  KS G A +  DL++  ML A V   P  G   
Sbjct: 178 PPSAPALKPASQFRLIGRDGVKRSDSRVKSDGTAQFGADLRLPGMLTALVARPPVWGARP 237

Query: 248 GSLRNQSQVEGMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQVDWQEAAADSALRVMPA 307
               ++     + GV +V  +P  +AV A  +W A +  EA+   W E A   +   +  
Sbjct: 238 DG-HDRKAALAVPGVMAVFPIPSGIAVAASSFWAAHKGREALNARWAEPADKVSTESLRR 296

Query: 308 DFSSDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEATYHNQYLNHAQLEPPSALAR 367
           D+++      L  + G  R     GD    L  A  +  ATY   YL HA +EP + + R
Sbjct: 297 DWTA------LLGKAG--RPAAARGDAEAILAQAAQRQSATYELPYLAHAPMEPLNCVVR 348

Query: 368 FNPDGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPLLGGFFGRHFLYDSANPYP-- 425
             P G  EIW   Q     +A  AK  GL P Q+ +H+ L GG FGR      ANP    
Sbjct: 349 LAP-GKCEIWTGCQFQTHDQAMAAKAAGLKPEQVEIHTMLAGGSFGRR-----ANPTSDY 402

Query: 426 --QAIALAKAVSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDDKGLPVA----IEAVSA 479
             + + +AK ++ P++L+W+RE++      RP+A+  F AALD++G+P+A    +   S 
Sbjct: 403 IVEGVEVAKVLNTPVRLMWTREDDIAGAYYRPMALHGFEAALDERGMPLAWRHRVVCQSI 462

Query: 480 TEGPTEALAGKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPMLGYWRSVGNSLNDF 539
            +G     AG   D IDP +VEG S   YAIPN  +     K    + +WRSVG+S   F
Sbjct: 463 LDGGP--FAGMIKDGIDPVSVEGASNLPYAIPNLAVDLHSPKAPVPVLWWRSVGSSHTAF 520

Query: 540 FYESFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWKRGPFTAEDGTRRAR 599
             E FLDELA  GG DP ELR  LL D PR   +L+ A     GW   P  A  G    R
Sbjct: 521 ATECFLDELAQAGGKDPLELRRALLADQPRHLAVLELAAA-KAGWG-SPLPAGKG----R 574

Query: 600 GVAMASPFGSHTAAIAEVSI-ENGKVKVHDIWQAIDPGSIVNPAIVEAQVNGAVALGLSQ 658
           G+A+   F S  A + EVSI   G++KV  +  A+D G  V P +V AQ+ G +A  LS 
Sbjct: 575 GIAVHESFHSFVAQVVEVSIGAKGEIKVDRVVCAVDCGVAVTPDVVRAQMEGGIAFALSA 634

Query: 659 TLLEEAVYVDGKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIGEPPLPAVAPAVAN 718
            L  E    DG+    N+  Y  L   +M RV V +V S     G+GEP +P +APA+AN
Sbjct: 635 ALFGEITLKDGRAEQSNFHDYRCLRMNEMPRVEVHIVPSAAPPTGVGEPGVPPLAPALAN 694

Query: 719 AVAQLTGQRVRSLPL 733
           A+   TG+R+R LPL
Sbjct: 695 AIFAATGKRLRKLPL 709


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1373
Number of extensions: 68
Number of successful extensions: 11
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 713
Length adjustment: 40
Effective length of query: 699
Effective length of database: 673
Effective search space:   470427
Effective search space used:   470427
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources