Alignments for a candidate for fumD in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Magnetospirillum magneticum AMB-1

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate WP_011383741.1 AMB_RS06760 fumarate hydratase

Query= BRENDA::P0AC33
         (548 letters)



>NCBI__GCF_000009985.1:WP_011383741.1
          Length = 538

 Score =  732 bits (1890), Expect = 0.0
 Identities = 355/532 (66%), Positives = 425/532 (79%)

Query: 6   FHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPA 65
           F +   FPL  DDT Y  +T+E V  + F GQ ++ VAPEA+T L ++A  D S +LRPA
Sbjct: 4   FAFHELFPLSHDDTPYRKITAEGVGTASFNGQSVVTVAPEAITALTKEAIKDTSHLLRPA 63

Query: 66  HQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGG 125
           H QQ+ +IL D  +S ND++VA   L+N+ IA+ GVLP CQDTGTAI++GKKGQRVWTGG
Sbjct: 64  HLQQLRNILDDAGSSPNDRFVAYDLLKNACIASGGVLPMCQDTGTAIVMGKKGQRVWTGG 123

Query: 126 GDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAK 185
            DEAA+++GV   Y E NLRYSQ APLDMY+EVNTGTNLPAQIDLYA +G+ YKF+ +AK
Sbjct: 124 NDEAAISKGVQEAYTELNLRYSQTAPLDMYEEVNTGTNLPAQIDLYATEGEAYKFMFMAK 183

Query: 186 GGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTV 245
           GGGSANKT+LYQ+TKALL P  L ++L  ++RTLGT+ACPPYH+A VIGG SAE  LKTV
Sbjct: 184 GGGSANKTFLYQQTKALLNPKSLLSFLDAQIRTLGTSACPPYHLAIVIGGLSAEMCLKTV 243

Query: 246 KLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPR 305
           KLASAKY D LPT GN+ GQAFRD+ELE+ +L   Q++G+GAQFGGKYF HD+RVIRLPR
Sbjct: 244 KLASAKYLDSLPTAGNKFGQAFRDLELEQIVLKMTQDMGIGAQFGGKYFCHDVRVIRLPR 303

Query: 306 HGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNR 365
           HGASCPV +GVSCSADR I  KI + G+++E +E +P KY+P+       GE V++DLNR
Sbjct: 304 HGASCPVAIGVSCSADRQILGKITKDGVYVEAMEKDPAKYLPDVDPSKLAGEVVKIDLNR 363

Query: 366 PMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPA 425
           PM EI   LSQYPV TR+SL G +IV RDIAHAKLKER+D GEGLP+Y K   +YYAGPA
Sbjct: 364 PMDEIRKTLSQYPVKTRVSLTGPMIVARDIAHAKLKERLDRGEGLPEYFKQMGVYYAGPA 423

Query: 426 KTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSI 485
           KTP  +ASGS GPTTAGRMDSYVDQ QA GGSM+M+AKGNRS+ VT+ACKKHGGFYLGSI
Sbjct: 424 KTPSNFASGSFGPTTAGRMDSYVDQFQAAGGSMLMIAKGNRSKAVTEACKKHGGFYLGSI 483

Query: 486 GGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQ 537
           GG AA LAQ  IK +E +EYPELGMEAIW+IEV DFPAFI+VDDKGNDFF +
Sbjct: 484 GGAAARLAQDCIKKVEVIEYPELGMEAIWRIEVVDFPAFIVVDDKGNDFFAE 535


Lambda     K      H
   0.317    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 957
Number of extensions: 42
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 538
Length adjustment: 35
Effective length of query: 513
Effective length of database: 503
Effective search space:   258039
Effective search space used:   258039
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources