Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate WP_043743905.1 AMB_RS08470 ABC transporter ATP-binding protein
Query= uniprot:Q1MCU3 (247 letters) >NCBI__GCF_000009985.1:WP_043743905.1 Length = 234 Score = 219 bits (557), Expect = 5e-62 Identities = 112/232 (48%), Positives = 164/232 (70%), Gaps = 1/232 (0%) Query: 11 LLQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVF 70 LL+++G+ ++YG I+AL G+DV V +GE+V+L+GANGAGK+TL+ I G GS+ F Sbjct: 2 LLEISGLTSHYGRIQALKGIDVQVAEGELVALVGANGAGKTTLLRCISGVQGVTGGSIKF 61 Query: 71 EGRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKHFAEDVEKIFTLF 130 G+DIT+M L I+QSPEGR++F M+V +NL++GA + D+++++ +F Sbjct: 62 AGQDITKMSPEARVGLGISQSPEGRQVFGPMSVEDNLRLGAYRRHGPDIKADMDRMYEMF 121 Query: 131 PRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKLN 190 P L ++ GTLSGG+QQML+I RALMA P++LLLDEPS+GLAPL+V IF I+ L Sbjct: 122 PILHKKRELPAGTLSGGQQQMLAIARALMANPRVLLLDEPSMGLAPLLVAEIFRTIQALK 181 Query: 191 EAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAYL 242 + G+T+FLVEQNA+AAL ++ R YV+ G V ++ G LL + +V+ AYL Sbjct: 182 DG-GMTIFLVEQNAYAALAIADRGYVLETGAVVLTDQGSRLLNDDKVKEAYL 232 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 208 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 234 Length adjustment: 23 Effective length of query: 224 Effective length of database: 211 Effective search space: 47264 Effective search space used: 47264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory