Alignments for a candidate for ech in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Magnetospirillum magneticum AMB-1

Align enoyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate WP_011385366.1 AMB_RS15050 multifunctional fatty acid oxidation complex subunit alpha

Query= BRENDA::P07896
         (722 letters)



>NCBI__GCF_000009985.1:WP_011385366.1
          Length = 703

 Score =  441 bits (1133), Expect = e-128
 Identities = 262/707 (37%), Positives = 394/707 (55%), Gaps = 27/707 (3%)

Query: 5   LRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTVK----AIVICGANGNFCAG 60
           L +   +A + + +PPVNA        VR GLQK  +D   +    A+++  A   F AG
Sbjct: 7   LSVSDRIATVTIDSPPVNAAD----HPVRAGLQKVFTDLAARSDYDAVLVLCAGRTFMAG 62

Query: 61  ADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQGVALGGGLELALGCHYRIANAKARVG 120
           ADI  F           L + ++   KPV+AA+ G ALG G ELA+ CHYRIA+  AR+G
Sbjct: 63  ADIGEFDTGIKAPHHQDLFNLVENCAKPVVAALHGTALGAGTELAMACHYRIADKGARIG 122

Query: 121 LPEVTLGILPGARGTQLLPRVVGVPVALDLITSGKYLSADEALRLGILDAVVKSDPVEEA 180
           LPE++LGI+PGA GTQ  PR++G+  A+DL+ SGK L A +A  LG++D +   D    A
Sbjct: 123 LPELSLGIIPGAGGTQRAPRLIGLDAAMDLVLSGKPLPAPKAAELGLVDEIASGDLNAAA 182

Query: 181 IKFAQKIIDKPIEPRRIFNKPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASV 240
           + FA+K++ +    RR  +           + A   A+V K      +P   + ++ A+ 
Sbjct: 183 LAFAKKLVAEGKGVRRTCDMAPKDAAKDAEIIAARQAQVAKTMKNRTSPLKALEAMAATT 242

Query: 241 KHPYEVGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQPVSSVG 300
              +  G++ E  +   L  + +A+A+++ FFA++   K    +    K   A+P+  VG
Sbjct: 243 TLSFAEGLRLEADISKQLEHAVEARAMRHLFFADREVRKIPGIT----KDIKARPIRKVG 298

Query: 301 VLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEKEASRAH--QNGQASA 358
           ++G GTMG GIA+ FA +GI V  ++   + L     +I    E+  SR    Q    S 
Sbjct: 299 IIGAGTMGGGIAMCFANIGIPVTIIDVSDENLQRGLGVIRKNYERSVSRGSLTQEQLESR 358

Query: 359 KPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALNVDDIA 418
              L  S+    L   DL +EAVFE M LKK +FA+L A+   GA L TNTS L++D+IA
Sbjct: 359 MGLLSASTDYAALKDADLAIEAVFEKMELKKDIFAKLDAVLPAGAILGTNTSTLDIDEIA 418

Query: 419 SSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGF 478
           ++T RP  VIG HFFSPA+VM LLE++  + ++   + T + ++K I K GVV   CYGF
Sbjct: 419 NTTKRPADVIGLHFFSPANVMPLLEIVQGKQTAMDVLLTALDMAKLIKKTGVVSKVCYGF 478

Query: 479 VGNRMLAPYYNQGFFLLEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGL 538
           +GNRM+ PY  +   ++ EG+ P ++DG LE +G  MG   V D+AG++VG   R    +
Sbjct: 479 IGNRMMDPYGREAERMVLEGATPAEIDGALEGWGMAMGILAVYDMAGVEVGDNTR----I 534

Query: 539 TGPSLPPGTPVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTF 598
             P + P  P   R ++       +L      GQK G+G+Y+YD P  R    DP +   
Sbjct: 535 ANPHMVPDDPSFYRCST-------LLVANKWLGQKVGRGYYRYDSP-DRKRASDPEVIEM 586

Query: 599 LSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDVIYLHGYGWPRH 658
           +    +  ++  R   K+EILERCLYS+INE  ++LEEG+A R   IDV+Y  GYG+PR+
Sbjct: 587 MHAEGKRLNVPARKPGKDEILERCLYSMINEGAKLLEEGIALRASDIDVVYTAGYGFPRY 646

Query: 659 KGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQGS 705
           +GGPMFYA ++GL  + +K+ + +++  D     P+  L +L   GS
Sbjct: 647 RGGPMFYADTIGLKVIYDKIVE-FQKTLDPRYWTPAPLLEKLAKAGS 692


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1195
Number of extensions: 69
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 722
Length of database: 703
Length adjustment: 39
Effective length of query: 683
Effective length of database: 664
Effective search space:   453512
Effective search space used:   453512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources