Align LacK, component of Lactose porter (characterized)
to candidate WP_011385763.1 AMB_RS17210 molybdenum import ATP-binding protein ModC
Query= TCDB::Q01937 (363 letters) >NCBI__GCF_000009985.1:WP_011385763.1 Length = 363 Score = 133 bits (335), Expect = 7e-36 Identities = 77/224 (34%), Positives = 126/224 (56%), Gaps = 11/224 (4%) Query: 8 DIRKSYGSLEVIKGVNLEVSSGEFVVFVGPSGCGKSTLLRMIAGLEDISSGELTIGGTVM 67 D+R+ G + V L G ++ G SG GK++++ M+AGL G +++ G V+ Sbjct: 5 DLRRRQGEFRL--DVRLSAGPGVTALY-GRSGSGKTSVINMVAGLSRPDEGSISVDGRVL 61 Query: 68 ND------VDPSKRGIAMVFQTYALYPHMTVRENMGFALRFAGMAKDEIERRVNAAAKIL 121 D + P R + VFQ + L+PH++VR N+ F + A E + ++ ++L Sbjct: 62 FDSRSGIDLPPEARRLGYVFQEHRLFPHLSVRGNLEFGQKLLPSA--ERTQSLDKVVELL 119 Query: 122 ELDALMDRKPKALSGGQRQRVAIGRAIVRQPDVFLFDEPLSNLDAELRVHMRVEIARLHK 181 +++L+DR+P LSGG++QRVAIGRA++ P + L DEPL+ LD + + IA+L + Sbjct: 120 GIESLLDRRPAKLSGGEKQRVAIGRALLASPRILLMDEPLAALDPARKAEVLPFIAQLAR 179 Query: 182 ELNATIVYVTHDQVEAMTLADKIVVMRGGIVEQVGAPLALYDDP 225 + I+YV+H E + LAD + +M GG V G +L DP Sbjct: 180 RFSVPILYVSHSMDEVLRLADTLALMDGGKVAASGPLESLMGDP 223 Lambda K H 0.320 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 326 Number of extensions: 17 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 363 Length of database: 363 Length adjustment: 29 Effective length of query: 334 Effective length of database: 334 Effective search space: 111556 Effective search space used: 111556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory