Align Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (characterized)
to candidate WP_011383120.1 AMB_RS03480 isovaleryl-CoA dehydrogenase
Query= reanno::Smeli:SM_b21121 (387 letters) >NCBI__GCF_000009985.1:WP_011383120.1 Length = 389 Score = 617 bits (1591), Expect = 0.0 Identities = 309/386 (80%), Positives = 331/386 (85%) Query: 2 FEAGLNFALGEEIDALRASVRRFASERIAPLADDADRSNAFPMSLWREMGELGLLGITAD 61 F + LNF LGE D +R SV FA+ IAP A + DRSN FP LW +G +GLLGIT D Sbjct: 4 FNSALNFDLGETADMMRDSVAAFAATEIAPRAAEIDRSNEFPNELWPRLGAMGLLGITVD 63 Query: 62 EAHGGAGLGYLAHCVAMEEISRASASVGLSYGAHSNLCVNQINRNGKPAQKSRYLPKLIS 121 E +GGAG+GYL H VAMEEISRASASVGLSYGAHSNLCVNQI RNG AQK +YLPKLIS Sbjct: 64 EKYGGAGMGYLEHVVAMEEISRASASVGLSYGAHSNLCVNQIKRNGTEAQKMKYLPKLIS 123 Query: 122 GEHVGALAMSEPGAGSDVVSMKLKADKRGDRYVLNGSKMWITNGPDADVLVVYAKTDPAA 181 GE++GALAMSEP AGSDVVSMKLKA+K+GDRYVLNG+KMWITNGPDADV+VVYAKTD A Sbjct: 124 GEYIGALAMSEPNAGSDVVSMKLKAEKKGDRYVLNGTKMWITNGPDADVIVVYAKTDVTA 183 Query: 182 GPRGITAFLVEKAFPGFSAGQKLDKLGMRGSNTSELIFTDCEVPEENVLGGVGEGVKVLM 241 GPRGITAFLVEK F GFS QKLDKLGMRGSNT EL+F DCEVPEENVLG VG+GV VLM Sbjct: 184 GPRGITAFLVEKTFKGFSVAQKLDKLGMRGSNTGELVFQDCEVPEENVLGAVGKGVNVLM 243 Query: 242 SGLDYERVVLSAGPLGIMAACLDVVVPYLHERKQFGQPIGEFQLMQGKLADMYVTMNAAR 301 SGLDYERVVL+ GPLGIMAAC+DVVVPY+HER QFGQPIG FQLMQGKLADMY T NA R Sbjct: 244 SGLDYERVVLTGGPLGIMAACMDVVVPYVHERTQFGQPIGTFQLMQGKLADMYTTFNACR 303 Query: 302 AYVYAVAAACDRGETARKDAAGCILYAAEKATAMALEAIQALGGNGYTNDYPAGRLLRDA 361 AY YAVA ACDRGET+RKDAAG ILY AEKAT MALEAIQ LGGNGY N+Y GRLLRDA Sbjct: 304 AYTYAVAKACDRGETSRKDAAGVILYGAEKATWMALEAIQTLGGNGYINEYATGRLLRDA 363 Query: 362 KLYEIGAGTSEIRRMLIGRELFAETK 387 KLYEIGAGTSEIRRMLIGRELF ETK Sbjct: 364 KLYEIGAGTSEIRRMLIGRELFEETK 389 Lambda K H 0.318 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 475 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 387 Length of database: 389 Length adjustment: 30 Effective length of query: 357 Effective length of database: 359 Effective search space: 128163 Effective search space used: 128163 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory