GapMind for catabolism of small carbon sources

 

Alignments for a candidate for badH in Magnetospirillum magneticum AMB-1

Align BadH (characterized)
to candidate WP_011384971.1 AMB_RS13035 NAD(P)-dependent oxidoreductase

Query= metacyc::MONOMER-893
         (255 letters)



>NCBI__GCF_000009985.1:WP_011384971.1
          Length = 248

 Score =  232 bits (592), Expect = 5e-66
 Identities = 118/253 (46%), Positives = 164/253 (64%), Gaps = 6/253 (2%)

Query: 3   RLQNKTAVITGGGGGIGGATCRRFAQEGAKIAVFDLNLDAAEKVAGAIRDAGGTAEAVRC 62
           + ++K A+ITGG  GIG  T +  A+ GA + + D+N++A EK A  +   G  AE VR 
Sbjct: 2   KFEDKVALITGGASGIGYCTVKSMAELGADVLIADINVEAGEKAAAELTAKGFKAEFVRL 61

Query: 63  DIADRTSVDAAIATTTTTLGPVDILVNNAGWDIFKPFTKTEPGEWERLIAINLTGALHMH 122
           D+ D+ ++         T G +DIL N AGW   +PF   +     +++++NLTG + + 
Sbjct: 62  DVTDKANIARVKEHVVATRGRLDILCNVAGWGHIQPFVDNDDAFIAKVMSLNLTGPIELI 121

Query: 123 HAVLPGMVERRHGRIVNIASDAARVGSSGEAVYAACKGGLVAFSKTLAREHARHGITVNV 182
            A  P M+E++ G+IVN+ASDA RVGS GE+VY+A KGGL+AFSK LARE AR  I VN 
Sbjct: 122 RAFFPLMIEKKTGKIVNVASDAGRVGSLGESVYSAAKGGLIAFSKALAREGARFNINVNA 181

Query: 183 VCPGPTDTALLADVTSGAANPEKLIEAFTKAIPLGRLGKPDDLAGAIAFFGSDDAGFITG 242
           +CPGPTDT LL       + PEK +EAF K IP+ R G+P ++A +I F  S+ A +ITG
Sbjct: 182 ICPGPTDTPLL------KSEPEKFLEAFLKVIPMRRFGQPQEVADSIVFMASNRADYITG 235

Query: 243 QVLSVSGGLTMNG 255
           QVLSV+GG+TM G
Sbjct: 236 QVLSVNGGITMVG 248


Lambda     K      H
   0.318    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 192
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 248
Length adjustment: 24
Effective length of query: 231
Effective length of database: 224
Effective search space:    51744
Effective search space used:    51744
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory