GapMind for catabolism of small carbon sources

 

Alignments for a candidate for livG in Magnetospirillum magneticum AMB-1

Align High-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG (characterized, see rationale)
to candidate WP_011384430.1 AMB_RS10235 ABC transporter ATP-binding protein

Query= uniprot:A0A159ZWS6
         (255 letters)



>NCBI__GCF_000009985.1:WP_011384430.1
          Length = 245

 Score =  193 bits (491), Expect = 2e-54
 Identities = 106/250 (42%), Positives = 152/250 (60%), Gaps = 14/250 (5%)

Query: 5   ILKVENLSMRFGGLLAVNGVALTVKEKQVVALIGPNGAGKTTVFNCLTGFYQPTGGTILL 64
           +L VENL   F GL AVN VA  V    +VALIGPNGAGKTT FN + G   P+ G ++L
Sbjct: 7   MLSVENLGKSFRGLRAVNQVAFEVPRGDIVALIGPNGAGKTTTFNLIAGALPPSDGKVVL 66

Query: 65  DGEPIQGLPGHHIARKGVVRTFQNVRLFKDMTAVENLLIAQHRHLNTNFFAGLFKTPAFR 124
           DG  I GL    I R GV RTFQ V+ F  ++  EN+++   RH N +            
Sbjct: 67  DGHDITGLGQAAICRAGVGRTFQIVKPFAGLSVEENVMVGALRHDNGDV----------- 115

Query: 125 KSEREAMEYAEYWLDKVNLTEFANRPAGTLAYGQQRRLEIARCMMTRPRILMLDEPAAGL 184
               +A   +   LD + L + A  PA TL    ++RLE+A+ + TRP++L+LDE  AGL
Sbjct: 116 ---DKARAASRATLDLLELGDKARDPAQTLTLPDRKRLEVAKALATRPKLLLLDEVMAGL 172

Query: 185 NPKETEDLKALIGVLREEHNVTVLLIEHDMKLVMSISDHIVVINQGTPLADGTPEQIRDN 244
            P E + + A++  L +E  +T+LLIEH M+ VM++SDH+VV++ G  +A+GTP ++  +
Sbjct: 173 RPVECDRMVAILTGLNKETGLTILLIEHVMRAVMALSDHVVVLHHGEKIAEGTPAEVTSH 232

Query: 245 PEVIKAYLGE 254
           P+V++ YLGE
Sbjct: 233 PKVLECYLGE 242


Lambda     K      H
   0.321    0.138    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 245
Length adjustment: 24
Effective length of query: 231
Effective length of database: 221
Effective search space:    51051
Effective search space used:    51051
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory