Align Phenylacetyl-CoA:acceptor oxidoreductase small subunit PadC (characterized, see rationale)
to candidate WP_043744126.1 AMB_RS10175 4Fe-4S dicluster domain-containing protein
Query= uniprot:A0A2R4BLY8 (215 letters) >NCBI__GCF_000009985.1:WP_043744126.1 Length = 217 Score = 329 bits (844), Expect = 2e-95 Identities = 143/213 (67%), Positives = 174/213 (81%) Query: 1 MTRYAMVADLRRCVGCQTCTAACKHTNATPPGVQWRWVLDVEAGEFPDVSRTFVPVGCQH 60 MTRY MVADLRRCVGCQTCTA+CK TNATPPGVQWR VLD+EAG++PDV R F+P GCQH Sbjct: 1 MTRYVMVADLRRCVGCQTCTASCKETNATPPGVQWRKVLDIEAGQYPDVRRIFLPTGCQH 60 Query: 61 CDEPPCETVCPTTATKKRADGLVTIDYDLCIGCAYCSVACPYNARYKVNFAEPAYGDRLM 120 CD+PPC VCP+TAT+KR DG+VTIDYD+CIGC+YC+V+CPY ARYKV AE YG + Sbjct: 61 CDQPPCMEVCPSTATRKRDDGIVTIDYDICIGCSYCAVSCPYEARYKVESAEYVYGAGPI 120 Query: 121 ANEKQRADPARVGVATKCTFCSDRIDYGVAHGLTPGVDPDATPACANACIANALTFGDID 180 + EK RA ++GV TKCTFC +RID GV G PG+ P+ATPAC N+C++ AL FGDI+ Sbjct: 121 SQEKARAAQQKIGVVTKCTFCVERIDAGVKAGKVPGIHPEATPACVNSCLSGALRFGDIE 180 Query: 181 DPNSKASRLLRENEHFRMHEELGTGPGFFYLWE 213 DP+S SRL+ +N+ FR HE+ GTGPGF+YLW+ Sbjct: 181 DPDSVVSRLVGDNKWFRQHEDEGTGPGFYYLWD 213 Lambda K H 0.323 0.137 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 293 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 215 Length of database: 217 Length adjustment: 22 Effective length of query: 193 Effective length of database: 195 Effective search space: 37635 Effective search space used: 37635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory