GapMind for catabolism of small carbon sources

 

Aligments for a candidate for AZOBR_RS08245 in Magnetospirillum magneticum AMB-1

Align Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate WP_011385946.1 AMB_RS18160 ABC transporter ATP-binding protein

Query= uniprot:G8ALJ0
         (294 letters)



>lcl|NCBI__GCF_000009985.1:WP_011385946.1 AMB_RS18160 ABC
           transporter ATP-binding protein
          Length = 265

 Score =  198 bits (503), Expect = 1e-55
 Identities = 111/271 (40%), Positives = 169/271 (62%), Gaps = 8/271 (2%)

Query: 5   SMTTTPLLTVEHLTMRFGGLVAVNDVSFSANNGEITAIIGPNGAGKTTLFNCITGFYTPT 64
           S+T  P+L V  +++ FGG+ A+ +VSF  N GE+ +IIGPNGAGKT++ NC++G Y PT
Sbjct: 2   SVTDNPVLEVRDVSLAFGGVRALTEVSFQVNKGELFSIIGPNGAGKTSMLNCVSGRYKPT 61

Query: 65  VGRLTLRHADGKEFLLERMPGYRISQKASVARTFQNIRLFGGMSVLENLIVAQHNKLIRA 124
            GR+ +   DG++ +    P  R +    + RTFQN+ LFG MSVL+N++V +H+ L   
Sbjct: 62  SGRVFM---DGRD-VTGLSPNVRAT--LGLGRTFQNLALFGHMSVLDNIMVGRHHLL--G 113

Query: 125 SGFSIAGLLGLPSYTRTEREAVDLAKYWLDRVRLLEFADWEAGNLPYGAQRRLEIARAMC 184
           +  +   L  L    + E       +  +D + +       AG LPYG ++R+E+ARAM 
Sbjct: 114 NNAATGALYWLSGARKEELAHRRKVEDIIDFLEIAHIRKAVAGTLPYGLRKRVELARAMA 173

Query: 185 TEPVMLCLDEPAAGLNPRESGELADLLTYIRDEHKIGVLLIEHDMSVVMTISDHVVVLDY 244
            EP ++ LDEP AG+N  E  ++A  +  + +E  + V++IEHDM VVM IS  V+VL++
Sbjct: 174 VEPKVILLDEPMAGMNLEEKEDMARYIVDLNEEWGMTVIMIEHDMGVVMDISHRVMVLEF 233

Query: 245 GRKISDGDPAFVKNDPAVIRAYLGEEEDEEL 275
           GRKI++G P  V N+  V  AYLG +EDE++
Sbjct: 234 GRKIAEGLPDEVMNNERVKVAYLGIDEDEDV 264


Lambda     K      H
   0.319    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 196
Number of extensions: 13
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 294
Length of database: 265
Length adjustment: 26
Effective length of query: 268
Effective length of database: 239
Effective search space:    64052
Effective search space used:    64052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory