Align Monocarboxylic acid transporter (characterized)
to candidate WP_011385006.1 AMB_RS13215 cation acetate symporter
Query= SwissProt::Q8NS49 (551 letters) >NCBI__GCF_000009985.1:WP_011385006.1 Length = 554 Score = 413 bits (1061), Expect = e-119 Identities = 237/543 (43%), Positives = 325/543 (59%), Gaps = 39/543 (7%) Query: 23 ISVFVVFIIVTMTVVLRVGKSTSESTDFYTGGASFSGTQNGLAIAGDYLSAASFLGIVGA 82 I++F F++ T+ + K T ++DFYT G +G QNGLAIAGDY+SAA+ LG+ Sbjct: 37 IAMFFAFVMGTLGITYWASKRTKSASDFYTAGGGITGFQNGLAIAGDYMSAATLLGLSSM 96 Query: 83 ISLNGYDGFLYSIGFFVAWLVALLLVAEPLRNVGRFTMADVLSFRLRQKPVRVAAACGTL 142 + G+DGF+Y+I FFV W + L L+AE LRN+GR+T AD+ S+RL Q VR AA G+L Sbjct: 97 VFATGFDGFIYTISFFVGWPIILFLLAERLRNLGRYTFADIASYRLDQSKVRTFAAMGSL 156 Query: 143 AVTLFYLIAQMAGAGSLVSVLLDIHEFKWQAVVVGIVGIVMIAYVLLGGMKGTTYVQMIK 202 V FYLI QM GAG L+ +L + AV V IVG++M+ YV GGM TT+VQ+IK Sbjct: 157 TVVCFYLIVQMVGAGQLIKLLFGLD----YAVAVIIVGVLMVVYVTFGGMIATTWVQIIK 212 Query: 203 AVLLVGGVAIMTVLTFVKVSGGLTTLLNDAVEKHAASDYAATKGYDPTQILEPGLQYGAT 262 A LL+GG ++ L K L + AVE H A +I+ P G Sbjct: 213 ACLLLGGGVLLCALALSKFGFSLENVAAKAVESHKAG----------VKIMGP----GTM 258 Query: 263 LTTQLDFISLALALCLGTAGLPHVLMRFYTVPTAKEARKSVTWAIVLIGAFYLMTLVLGY 322 L + +SL+L L GTA LPH+LMRF+TVP AKEARKSV A IG F+L+ +LG Sbjct: 259 LADPVSAVSLSLGLVFGTAALPHILMRFFTVPNAKEARKSVFVASGFIGFFFLIVCILGL 318 Query: 323 GAAALVGPDRVI--------AAPGAANAAAPLLAFELGGSIFMALISAVAFATVLAVVAG 374 A ++VG D G N LA LGG IF+ +SAVAFAT+LAVV+G Sbjct: 319 AAVSIVGTDPQFFEGGKVGGKLLGGGNMPVMHLAKALGGDIFLGFLSAVAFATILAVVSG 378 Query: 375 LAITASAAVGHDIYNAVIRNGQSTEAEQVRVSRITVVVIGLISIVLGILAMTQNVAFLVA 434 LA+ ++A+ HDIY VIR G +TEAE++RVS+++ +V+G+++++LGI+ QNVAFLV Sbjct: 379 LALAGASAIAHDIYARVIRKGHATEAEEMRVSKLSSLVLGVVAVILGIMFEKQNVAFLVG 438 Query: 435 LAFAVAASANLPTILYSLYWKKFNTTGAVAAIYTGLISALLLIFLSPAVSGNDSAMVPGA 494 L F +AASAN P + S+YW+ T GA+ GL+SA+ + LS AV +V Sbjct: 439 LTFGIAASANFPVLFLSMYWRGLTTKGALWGGIAGLVSAVTCVVLSKAV----WVVVLNN 494 Query: 495 DWAIFPLKNPGLVSIPLAFIAGWIGTLVGKPDNMDDLAAE------MEVRSLTGVGVEKA 548 IFP ++P L S+ LAF+ + +V K D + AE VR+ TG+G A Sbjct: 495 PAPIFPYEHPALFSMTLAFL---VTIVVSKLDGSETAKAEKALFDDQYVRAQTGIGAASA 551 Query: 549 VDH 551 +H Sbjct: 552 SNH 554 Lambda K H 0.324 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 661 Number of extensions: 30 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 551 Length of database: 554 Length adjustment: 36 Effective length of query: 515 Effective length of database: 518 Effective search space: 266770 Effective search space used: 266770 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory