Align C4-dicarboxylate TRAP transporter large permease protein DctM (characterized)
to candidate WP_011383335.1 AMB_RS04550 C4-dicarboxylate ABC transporter permease
Query= SwissProt::O07838 (440 letters) >NCBI__GCF_000009985.1:WP_011383335.1 Length = 427 Score = 654 bits (1687), Expect = 0.0 Identities = 335/440 (76%), Positives = 378/440 (85%), Gaps = 14/440 (3%) Query: 1 MSALIIFGLLIALMLTGMPISISLGLTVLTFLFTMTQVPIDTVALKLFTGIEKFEIMAIP 60 M++ +IFGLLI LMLTGMPISISLGLTVL+FLF TQVPI++VALKLFTGIEKFEIMAIP Sbjct: 1 MNSAVIFGLLIVLMLTGMPISISLGLTVLSFLFLFTQVPIESVALKLFTGIEKFEIMAIP 60 Query: 61 FFILAGNFLTHGGVAKRMINFATAMVGHWHGGLGLAGVIACALFAAVSGSSPATVVAIGS 120 FFILAGNFLTHGGVA+RMINFATAMVGHW+GGLGLAGV+ACALFAAVSGSSPATVVAIGS Sbjct: 61 FFILAGNFLTHGGVARRMINFATAMVGHWYGGLGLAGVMACALFAAVSGSSPATVVAIGS 120 Query: 121 VILPAMVNQGFPKQFGAGVITTSGALGILIPPSIVMVMYAVATSGMVVTGPDGQPVSSAS 180 +ILPAMV QGFP +FGAGVITTSGALGILIPPS+VMVMYAVAT+ S Sbjct: 121 IILPAMVKQGFPNKFGAGVITTSGALGILIPPSLVMVMYAVATN--------------TS 166 Query: 181 VGELFMAGVVPGLMLAGFLAFTTWNRARKFGYPRLEKASLRQRWTAFREAAWGLMLIVVV 240 VG LFMAGV+PGL+LA L TW A K YPRL A+ QR+ AFREA WGL LIV+V Sbjct: 167 VGALFMAGVIPGLVLATVLGAVTWYIAWKNDYPRLPPATWAQRFRAFREAIWGLALIVIV 226 Query: 241 IGGIYAGIFTPTEAAAMSAVYAFFISVFVYKDLTLRDVPRVLLSSANMSAMLLYIITNAV 300 IGGIY G+FTPTEAAAMSAVYAF I+VFVYKD+ L+ V ++LLSSA+MSAMLLYIITNAV Sbjct: 227 IGGIYTGVFTPTEAAAMSAVYAFLIAVFVYKDMPLKGVGKILLSSASMSAMLLYIITNAV 286 Query: 301 LFSFLMAHEGIPQALGEWMVNAGLSWWMFLIIVNILLLAAGNFMEPSSIVLIMAPILFPV 360 LFSF++A+E IP A+ +W+V L FL++VN+LLL AGNFMEPSSIVLIMAPILFPV Sbjct: 287 LFSFVLANENIPHAIADWIVGKELGVIAFLLVVNVLLLVAGNFMEPSSIVLIMAPILFPV 346 Query: 361 AVRLGIDPVHFGIMIVVNMEVGMCHPPVGLNLYVASGITKMGITELTVAVWPWLLTMLAF 420 A++LGIDP+HFGI+IVVNMEVGMCHPPVGLNLYVASGITKMGITELTVAVWPWLL ML F Sbjct: 347 AMKLGIDPIHFGILIVVNMEVGMCHPPVGLNLYVASGITKMGITELTVAVWPWLLAMLGF 406 Query: 421 LVLVTYVPAISLALPNLLGM 440 L++VTY P +S+ LP LG+ Sbjct: 407 LMVVTYWPPLSIWLPRALGV 426 Lambda K H 0.329 0.142 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 768 Number of extensions: 38 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 440 Length of database: 427 Length adjustment: 32 Effective length of query: 408 Effective length of database: 395 Effective search space: 161160 Effective search space used: 161160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory