Aligments for a candidate for glk in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Aligments for a candidate for glk in Magnetospirillum magneticum AMB-1

Align glucokinase (EC 2.7.1.1; EC 2.7.1.2; EC 2.7.1.8) (characterized)
to candidate WP_011384511.1 AMB_RS10655 glucokinase

Query= ecocyc::GLUCOKIN-MONOMER
         (321 letters)



>lcl|NCBI__GCF_000009985.1:WP_011384511.1 AMB_RS10655 glucokinase
          Length = 321

 Score =  259 bits (663), Expect = 5e-74
 Identities = 146/311 (46%), Positives = 183/311 (58%), Gaps = 4/311 (1%)

Query: 1   MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHK--VEVKD 58
           M++  LV D+GGT+AR AL     GE          DY      I+ YL EH   V  K 
Sbjct: 1   MSQMVLVADIGGTHARFALMG-PDGEAVNPVVLRCADYDGPAPAIKAYLAEHAGGVAPKG 59

Query: 59  GCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQ 118
           G  A+A  I GD + +TN  W FSIAE ++ +G   LE++NDFTAV++++  LK EHL+ 
Sbjct: 60  GAFAVASVIDGDRIELTNSPWRFSIAETRQAVGLQRLEVVNDFTAVALSVRHLKPEHLMS 119

Query: 119 FGGAEPVEGKPIAVYGAGTGLGVAHLV-HVDKRWVSLPGEGGHVDFAPNSEEEAIILEIL 177
            GG  P  G PIAV G GTGLGV+ L+      W +L  EGGHV  A  +E EA IL+ L
Sbjct: 120 VGGGMPEAGLPIAVLGPGTGLGVSALIPSASGEWTALATEGGHVTMAAATEREARILDRL 179

Query: 178 RAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLF 237
           R +  HVSAERVLSG GLVNLY+A+            P  IT+R L  SC   R A+ +F
Sbjct: 180 RTQFDHVSAERVLSGQGLVNLYQAVAALSGHQAVFSTPDVITKRGLDGSCPVSREAVEVF 239

Query: 238 CVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVY 297
             +MG   GNLAL+LG  GGVFIAGGI+PR  E F+ S FR  FE  GRF+ Y+  IP +
Sbjct: 240 FAMMGTVAGNLALSLGAKGGVFIAGGILPRMAEAFRLSSFRTRFEAHGRFQPYLAAIPTW 299

Query: 298 LIVHDNPGLLG 308
           LI H  P  +G
Sbjct: 300 LITHPLPAFVG 310


Lambda     K      H
   0.322    0.141    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 324
Number of extensions: 20
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 321
Length of database: 321
Length adjustment: 28
Effective length of query: 293
Effective length of database: 293
Effective search space:    85849
Effective search space used:    85849
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate WP_011384511.1 AMB_RS10655 (glucokinase)
to HMM TIGR00749 (glk: glucokinase (EC 2.7.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00749.hmm
# target sequence database:        /tmp/gapView.3827.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00749  [M=315]
Accession:   TIGR00749
Description: glk: glucokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    4.3e-97  311.1   0.0    5.2e-97  310.8   0.0    1.0  1  lcl|NCBI__GCF_000009985.1:WP_011384511.1  AMB_RS10655 glucokinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000009985.1:WP_011384511.1  AMB_RS10655 glucokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  310.8   0.0   5.2e-97   5.2e-97       1     313 [.       6     310 ..       6     313 .. 0.95

  Alignments for each domain:
  == domain 1  score: 310.8 bits;  conditional E-value: 5.2e-97
                                 TIGR00749   1 lvgdiGGtnarlalvevapgeieqvktyssedfpsleavvrvyleeakvelkdpikgcfaiatPiigdf 69 
                                               lv+diGGt+ar+al+      +++  ++++ d++   ++++ yl e++  +  p  g+fa+a+ i gd 
  lcl|NCBI__GCF_000009985.1:WP_011384511.1   6 LVADIGGTHARFALMGPDGEAVNP-VVLRCADYDGPAPAIKAYLAEHAGGVA-PKGGAFAVASVIDGDR 72 
                                               89************6655555555.59*********************9986.689************* PP

                                 TIGR00749  70 vrltnldWalsieelkqelalaklelindfaavayailalkeedliqlggakveesaaiailGaGtGlG 138
                                               ++ltn  W +si+e++q ++l++le++ndf+ava+++  lk e+l+ +gg  +e + +ia+lG+GtGlG
  lcl|NCBI__GCF_000009985.1:WP_011384511.1  73 IELTNSPWRFSIAETRQAVGLQRLEVVNDFTAVALSVRHLKPEHLMSVGGGMPEAGLPIAVLGPGTGLG 141
                                               ********************************************************************* PP

                                 TIGR00749 139 vatliqqsdgrykvlageGghvdfaPrseleillleylrkkygrvsaervlsGsGlvliyealskrkge 207
                                               v+ li+ + g + +la+eGghv +a   e e+ +l+ lr+++++vsaervlsG+Glv++y+a+    g+
  lcl|NCBI__GCF_000009985.1:WP_011384511.1 142 VSALIPSASGEWTALATEGGHVTMAAATEREARILDRLRTQFDHVSAERVLSGQGLVNLYQAVAALSGH 210
                                               ***************************************************************999976 PP

                                 TIGR00749 208 revsklskeelkekdiseaalegsdvlarralelflsilGalagnlalklgarGGvyvaGGivPrfiel 276
                                               + v        ++  i+++ l+gs++++r+a+e+f +++G++agnlal lga+GGv++aGGi+Pr+ e 
  lcl|NCBI__GCF_000009985.1:WP_011384511.1 211 QAV------FSTPDVITKRGLDGSCPVSREAVEVFFAMMGTVAGNLALSLGAKGGVFIAGGILPRMAEA 273
                                               654......45899******************************************************* PP

                                 TIGR00749 277 lkkssfraafedkGrlkellasiPvqvvlkkkvGllG 313
                                               ++ ssfr +fe  Gr++ +la iP  ++ +  + ++G
  lcl|NCBI__GCF_000009985.1:WP_011384511.1 274 FRLSSFRTRFEAHGRFQPYLAAIPTWLITHPLPAFVG 310
                                               ****************************998777666 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (315 nodes)
Target sequences:                          1  (321 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.18
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources