Alignments for a candidate for gcvP in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for gcvP in Magnetospirillum magneticum AMB-1

Align Probable glycine dehydrogenase (decarboxylating) subunit 2; EC 1.4.4.2; Glycine cleavage system P-protein subunit 2; Glycine decarboxylase subunit 2; Glycine dehydrogenase (aminomethyl-transferring) subunit 2 (uncharacterized)
to candidate WP_011383212.1 AMB_RS03940 glycine dehydrogenase (aminomethyl-transferring)

Query= curated2:Q9A354
         (524 letters)



>NCBI__GCF_000009985.1:WP_011383212.1
          Length = 499

 Score =  665 bits (1715), Expect = 0.0
 Identities = 331/496 (66%), Positives = 379/496 (76%), Gaps = 3/496 (0%)

Query: 22  TLTGARGLLQDEALIFELDGWNKTGVDLPPVTAAPSSDLNGLLRDAPIGLPGLSEPETVR 81
           T++G R L  +E LIFEL       VDLP         L    R   + LP LSEP+ VR
Sbjct: 6   TISGNRALQIEEKLIFELGNPGSVAVDLPEPAPFDLERLGDAPRRGRVALPDLSEPQVVR 65

Query: 82  HYVRLSQKNHAIDLALYPLGSCTMKHNPRLNEKMARLPGFSDIHPLQPQSTVQGALELMD 141
           HY RLSQKN+ ID   YPLGSCTMKHNPRL+EK+ARLPG +D+HPLQPQ TVQGALE++D
Sbjct: 66  HYTRLSQKNYGIDTGFYPLGSCTMKHNPRLSEKVARLPGLADLHPLQPQKTVQGALEVID 125

Query: 142 RLAHWLKTLTGMPAVALTPKAGAHGELCGLLAIRAAHEAAGNGHRKTVLAPTSAHGTNPA 201
            LAHWLK LTGMPAVA++P AGAHGE CGL+AIR+AHE  G GHRK VL P SAHGTNPA
Sbjct: 126 TLAHWLKALTGMPAVAMSPAAGAHGEWCGLMAIRSAHEDKGEGHRKRVLVPESAHGTNPA 185

Query: 202 TAAFVGYTVVEIAQTEDGRVDLADLESKLGDHVAAIMVTNPNTCGLFERDVVEIARLTHA 261
           +AA  GYTV  I   E+GRVDLA L++KLG  VA +M+TNPNTCGLFE ++VEIA   H 
Sbjct: 186 SAAMCGYTVDPIPALENGRVDLAALKAKLGPDVACLMLTNPNTCGLFETEIVEIAAAVHG 245

Query: 262 AGAYFYCDGANFNAIVGRVRPGDLGVDAMHINLHKTFSTPHGGGGPGAGPVVLSEALAPF 321
           AGAYFYCDGANFNAIVGRV+  DLG+DAMHINLHKTF+TPHGGGGPGAGP VLS ALA F
Sbjct: 246 AGAYFYCDGANFNAIVGRVKIADLGIDAMHINLHKTFATPHGGGGPGAGPTVLSAALAAF 305

Query: 322 APTPWLTHGDNGFELAEHAGDDDAKTAFGRMSAFHGQMGMYVRAYAYMLSHGADGLRQVA 381
            P P++ HG +G EL E  G  D    FGR+  FHGQ G++VRA AY+ S G+DGLRQ +
Sbjct: 306 VPVPYVVHGASGLELVE--GKRDGAKPFGRVKGFHGQFGVFVRALAYIQSMGSDGLRQAS 363

Query: 382 EDAVLNANYIKAQLKDVMSPAFPEGPCMHEALFDDSWLEGTGVTTLDFAKAMIDEGFHPM 441
            DAVLNANY+ A LKD +S +F +GPCMHEALFDD +L+ TGVTTLDFAKAMIDEG+HPM
Sbjct: 364 SDAVLNANYLLASLKDELSASF-DGPCMHEALFDDRFLKDTGVTTLDFAKAMIDEGYHPM 422

Query: 442 TMYFPLVVHGAMLIEPTETESKHELDRFIAALRALAGAAKAGDTERFKGAPFHAPLRRLD 501
           TMYFPLVVHGA+L+EPTETESK  LD+FIA ++ LA  AKAG  E FK AP   P RRLD
Sbjct: 423 TMYFPLVVHGALLMEPTETESKDTLDQFIAVVKGLAAKAKAGGVEDFKAAPRLTPRRRLD 482

Query: 502 ETQAARKPRLRWKPVA 517
           ET AAR P LRWK  A
Sbjct: 483 ETLAARSPVLRWKTAA 498


Lambda     K      H
   0.318    0.135    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 847
Number of extensions: 29
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 524
Length of database: 499
Length adjustment: 35
Effective length of query: 489
Effective length of database: 464
Effective search space:   226896
Effective search space used:   226896
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources