Align Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1; L-threonine/L-allo-threonine aldolase; EC 4.1.2.48 (uncharacterized)
to candidate WP_011384736.1 AMB_RS11815 serine hydroxymethyltransferase
Query= curated2:D3DKC4 (427 letters) >NCBI__GCF_000009985.1:WP_011384736.1 Length = 427 Score = 482 bits (1241), Expect = e-141 Identities = 243/413 (58%), Positives = 300/413 (72%), Gaps = 6/413 (1%) Query: 4 LFNTDAEIYEAIVKEYERQFYHLELIASENFTSLAVMEAQGSVMTNKYAEGLPHKRYYGG 63 L D E++ AI +E +RQ +ELIASEN S AV+EAQGSVMTNKYAEG P KRYYGG Sbjct: 14 LSERDPEVFAAITQELKRQQDQIELIASENIVSRAVLEAQGSVMTNKYAEGYPGKRYYGG 73 Query: 64 CEFVDIAEDLAIERAKALFDAEHANVQPHSGTQANMAVYMAVLKPGDTIMGMDLSHGGHL 123 CEFVDIAE LAI RA +F +ANVQP SG+QAN V+MA+L+PGDTIMGM L+ GGHL Sbjct: 74 CEFVDIAESLAISRACQIFGCSYANVQPSSGSQANQGVFMALLQPGDTIMGMSLAAGGHL 133 Query: 124 THGAKVNFSGKIYNAVYYGVHPETHLIDYDQLYRLAKEHKPKLIVGGASAYPRVIDWAKL 183 THGA N SGK + AV YGV + ID+ ++ LA+ H+PKLI+ G SAYPR ID+A+ Sbjct: 134 THGAAPNQSGKWFKAVQYGVRQQDSQIDFAEVEELARTHRPKLIIAGGSAYPRTIDFARF 193 Query: 184 REIADSVGAYLMVDMAHYAGLIAGGVYPNPVPYAHFVTSTTHKTLRGPRSGFILCK-KEF 242 R+IAD VGA+ MVDMAH+AGL+AGGVYPNP+P+AH VT+TTHKTLRGPR G IL + Sbjct: 194 RKIADEVGAFFMVDMAHFAGLVAGGVYPNPLPHAHVVTTTTHKTLRGPRGGMILSNDADI 253 Query: 243 AKDIDKSVFPGIQGGPLMHVIAAKAVAFKEAMSQEFKEYARQVVANARVLAEEFIKEGFK 302 K I+ ++FPGIQGGPLMHVIA KAVAF EA+ EFK YA+QVV NAR LA+ ++ G Sbjct: 254 GKKINSAIFPGIQGGPLMHVIAGKAVAFGEALKPEFKLYAKQVVDNARALADTLVRRGLD 313 Query: 303 VVSGGTDSHIVLLDLRDTGLTGREVEEALGKANITVNKNAVPFDPLPPVKTSGIRLGTPA 362 +VSGGTDSH++L+DLR LTG+ E +L A +T NKN +PFDP P TSG+RLGTPA Sbjct: 314 IVSGGTDSHLMLVDLRPKKLTGKAAEASLEHAGMTCNKNGIPFDPEKPTITSGVRLGTPA 373 Query: 363 MTTRGMKEDQMRIIARLISKVIKNIG----DEKVIE-YVRQEVIEMCEQFPLY 410 TTRG ++ + + LI V+ + D E R EV E+C +FP+Y Sbjct: 374 ATTRGFGVEEFKKVGELIGDVLDGLAANPEDNSAAEARARAEVAELCRRFPIY 426 Lambda K H 0.319 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 577 Number of extensions: 24 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 427 Length of database: 427 Length adjustment: 32 Effective length of query: 395 Effective length of database: 395 Effective search space: 156025 Effective search space used: 156025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory