Align Protein containing DUF162 (characterized, see rationale)
to candidate WP_011386480.1 AMB_RS20895 lactate utilization protein
Query= uniprot:E4PLR7 (223 letters) >NCBI__GCF_000009985.1:WP_011386480.1 Length = 216 Score = 185 bits (470), Expect = 5e-52 Identities = 100/220 (45%), Positives = 141/220 (64%), Gaps = 10/220 (4%) Query: 2 SARANILGKLRNSLAGTTP-RPDEFDERLVTAPWRYAPEDRIERLRSLMEAVHTEVHPCR 60 S R IL +L+ + P RPD AP Y R + ++++EA H +VH Sbjct: 3 SPRERILARLKAAPQTAAPLRPD-------WAPPAYDASARKAKFKAMLEASHADVHEVT 55 Query: 61 SDNWPELVAELLNKRNLTNLLCAPSKEHGRALQAYFEATEQKVELLAYDQPVEAWKEELF 120 + +WP+ + +L + + N++ AP+ GR L + + ELLAYD+PVE +KE L Sbjct: 56 AADWPQRLKTILANKGVGNMVYAPATPAGRQLADSWAGSGP--ELLAYDRPVEDFKEVLV 113 Query: 121 WSVEASLTGTLGGIAATGTLVLWPDCHEPRLMSLVPPVHIALLKASEIHDNLYDMMVAQD 180 +A+LT GGIA TG+LVLWP EPRLMSL+PP+H+AL++ S + D+L + + Q Sbjct: 114 AKADAALTTARGGIAQTGSLVLWPTSDEPRLMSLLPPIHVALVEESSLTDSLAETIRVQG 173 Query: 181 WAAGLPTNVLLVSGPSKTADIEQVLAYGAHGPRELIVLVL 220 WA+G+PTN +LVSGPSKTADIEQ LAYG HGP+ELIVL++ Sbjct: 174 WASGMPTNAVLVSGPSKTADIEQTLAYGVHGPKELIVLLI 213 Lambda K H 0.317 0.133 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 180 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 223 Length of database: 216 Length adjustment: 22 Effective length of query: 201 Effective length of database: 194 Effective search space: 38994 Effective search space used: 38994 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory