Alignments for a candidate for treEIIA in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for treEIIA in Magnetospirillum magneticum AMB-1

Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate WP_043743857.1 AMB_RS08150 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo3_N2E3:AO353_15995
         (844 letters)



>NCBI__GCF_000009985.1:WP_043743857.1
          Length = 757

 Score =  263 bits (671), Expect = 4e-74
 Identities = 192/593 (32%), Positives = 282/593 (47%), Gaps = 18/593 (3%)

Query: 236 EDAELAISTLAQLLADGCGEAVTPVAVVAPVVEAQEVSTKLLRGVCASAGSAFGYVVQVA 295
           E  E     LA+LL  G  E V    +V   V+   +    + GV  + G   G  V   
Sbjct: 149 ETLETVAMVLAELLVSG--ELVNRAELVP--VDGNALLPLRMEGVKLNGGVGVGVAVLHR 204

Query: 296 ERTLEMPEFAADQQLERESLERALMHATQALQRL--RDNAAGEAQADIFKAHQELLEDPS 353
            R       A D   E E L++AL     AL+ L  R         D+ + ++   ED  
Sbjct: 205 PRITIQRLVAEDTDAELERLKKALTGIKLALEELFSRPEMRDGEHRDVLETYRMFAEDKG 264

Query: 354 LLEQAQALIAEGKSAAFAWNSATEATATLFKSLGSTLLAERALDLMDVGQRVLKLILGVP 413
            L +    I  G +A  A     + T      +    L ER  D  D+  R+L+ + G  
Sbjct: 265 WLNRISEAIHTGLTAEAAVQKVHDDTRARMSQITDPYLRERMHDFEDLANRLLQHLAGGD 324

Query: 414 D----GVWELPDQAILIAEQLTPSQTAALDTGKVLGFATVGGGATSHVAILARALGLPAV 469
           D    G  +LP+ AILI   L P +    D  ++ G     G ATSHVAI+ARAL +P V
Sbjct: 325 DDQRPGAADLPEGAILIGRNLGPMELFDYDPKRLRGLVLEEGSATSHVAIVARALDIPVV 384

Query: 470 CGLPLQVLSLASGTRVLLDADKGELHLDPAVSVIEQLHAKRQQQRQRHQHELENAARAAV 529
             +   +  +     V+ D D G++ + P   + E       Q++ R           AV
Sbjct: 385 GRVKDVLDKIEPLDPVICDGDNGQVFIRPPDDIRETYIDAISQRQLRQAVYARLRELPAV 444

Query: 530 TRDGHHFEVTANVASLAETEQAMSLGAEGIGLLRSEFLYQQRSVAPSHDEQAGTYSAIA- 588
           TR      +  N   L + +     GA+GIGL R+E  +  RS  P    Q   Y  I  
Sbjct: 445 TRQDVRIGIHMNAGLLLDLQHLHDSGADGIGLYRTELPFMARSSLPDVAAQTLLYKKILD 504

Query: 589 RALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPFLGMRGIRLCLERPQLLREQFRAILSS 648
           +A G  + +V RTLDVGGDK L Y     E NP LG R IR+ L+RP ++R Q RA+L +
Sbjct: 505 QAEG--KPVVFRTLDVGGDKVLPYWNPYEEDNPALGWRSIRITLDRPAVMRSQLRALLRA 562

Query: 649 AGLARLHIMLPMVSQLSELRLARLMLEEE-----ALALGLRELPKLGIMIEVPAAALMAD 703
           A    L +M PM++++SE   ARL+L++E     A+   +    K+G M+EVPA AL  D
Sbjct: 563 AQGRELSVMFPMIAEVSEFVQARLILDKELERERAMGNPMPSRLKVGTMLEVPALALQLD 622

Query: 704 LFAPEVDFFSIGTNDLTQYTLAMDRDHPRLASQADSFHPSVLRLIASTVKAAHAHGKWVG 763
              P VDF SIG+NDLTQ+  A+DR +PR+A + DS  P+++R +         +G  + 
Sbjct: 623 ALLPMVDFVSIGSNDLTQFLFAVDRGNPRIAERYDSLAPAMVRFMRYVAAKCAQYGVTLS 682

Query: 764 VCGALASETLAVPLLLGLGVDELSVSVPLIPAIKAAIREVELSDCQAIAHQVL 816
           VCG +A   L    L+G+G+  LS+S P I  +K  +R +++   +A  + +L
Sbjct: 683 VCGEMAGRPLEAMALIGVGIRHLSLSAPNIGPVKTMVRSLDIGALEAYMNTLL 735


Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1222
Number of extensions: 50
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 844
Length of database: 757
Length adjustment: 41
Effective length of query: 803
Effective length of database: 716
Effective search space:   574948
Effective search space used:   574948
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources