Alignments for a candidate for bkdC in Magnetospirillum magneticum AMB-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for bkdC in Magnetospirillum magneticum AMB-1

Align Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; EC 2.3.1.168; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (uncharacterized)
to candidate WP_011384715.1 AMB_RS11715 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Query= curated2:P37942
         (424 letters)



>NCBI__GCF_000009985.1:WP_011384715.1
          Length = 427

 Score =  221 bits (564), Expect = 3e-62
 Identities = 146/428 (34%), Positives = 225/428 (52%), Gaps = 28/428 (6%)

Query: 5   QMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVGEE 64
           Q+ MP L  ++TEG ++KWL   GD V   D + E+ TDK   E  +   G + +++   
Sbjct: 4   QILMPALSPTMTEGNLAKWLKNEGDAVKSGDILCEIETDKATMEFEAVDEGVLGKILVAG 63

Query: 65  GQT-----------LQVGE---MICKIETEGANPAEQKQEQPAASEA---AENPVAKSAG 107
           G +           L+ GE    I  I    A PA  K   PAA+ A   A  PVA  +G
Sbjct: 64  GTSGVAVNTPIAVLLEEGEDASAISAISAISAAPAP-KAAAPAAAAAPVTAAAPVAAPSG 122

Query: 108 AADQPNKKRYSPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQNPEELK 167
            A   ++   SP   R+A +  +DL  V G+G  GRI + D++  I+ G  +   P    
Sbjct: 123 PAHGGDRVVASPLAKRIAKDGNVDLKAVKGSGPHGRIVKADVEAAIKAGPAK---PAAAP 179

Query: 168 TAAPAPKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVD 227
            A  AP + S P P   + +  +    +EIP + +RK IA  +  +K+ IPH +  ++ +
Sbjct: 180 AAIVAPAAKSAPAPAAASPFEPAF---EEIPNSSMRKVIARRLTEAKSTIPHFYLSIDCE 236

Query: 228 VTNMVAYRNSIKDSFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINI 287
           +  ++  R  +     +++ + L+   F V+AVA ALK+ P  N+ W  + I +  DI+I
Sbjct: 237 LDALLKVRADLNG---RSDAYKLSVNDFVVRAVALALKKAPAANASWGEEAIKRYTDIDI 293

Query: 288 SIAVATEDSLFVPVIKNADEKTIKGIAKDITGLAKKVRDGKLTADDMQGGTFTVNNTGSF 347
           S+AVAT   L  P++ +AD K +  I+ ++  LA K RDGKL  ++ QGG FT++N G F
Sbjct: 294 SVAVATPSGLITPIVHHADHKGLAEISNEMKSLAAKARDGKLKPEEFQGGGFTISNLGMF 353

Query: 348 GSVQSMGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDGLVCGRFL 407
           G  +   IIN PQ  IL V +  +RPVV   G +AV  ++   LS+DHRV+DG V   FL
Sbjct: 354 GIKEFAAIINPPQGCILAVGAGEQRPVV-KAGALAVATVMTCTLSVDHRVVDGAVGAEFL 412

Query: 408 GRVKQILE 415
              K+++E
Sbjct: 413 AAFKKLIE 420


Lambda     K      H
   0.312    0.129    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 411
Number of extensions: 25
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 424
Length of database: 427
Length adjustment: 32
Effective length of query: 392
Effective length of database: 395
Effective search space:   154840
Effective search space used:   154840
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources