GapMind for catabolism of small carbon sources

 

Definition of D-alanine catabolism

As rules and steps, or see full text

Rules

Overview: GapMind describes D-alanine catabolism via D-alanine dehydrogenase, which forms pyruvate. This reaction is part of the MetaCyc pathway for L-alanine catabolism via D-alanine (link). In principle, D-alanine might also be catabolized via racemization to L-alanine and transamination to pyruvate, but this is not described here.

Steps

Pf6N2E2_5402: ABC transporter for D-Alanine, substrate-binding component

Pf6N2E2_5403: ABC transporter for D-Alanine, permease component 2

Pf6N2E2_5404: ABC transporter for D-Alanine, permease component 1

Pf6N2E2_5405: ABC transporter for D-Alanine, ATPase component

AZOBR_RS08235: D-alanine ABC transporter, permease component 1

AZOBR_RS08240: D-alanine ABC transporter, permease component 2

AZOBR_RS08245: D-alanine ABC transporter, ATPase component 1

AZOBR_RS08250: D-alanine ABC transporter, ATPase component 2

AZOBR_RS08260: D-alanine ABC transporter, substrate-binding component

cycA: D-alanine:H+ symporter CycA

mctP: D-alanine transporter MctP

dadA: D-alanine dehydrogenase

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory