GapMind for catabolism of small carbon sources

 

D-lactate catabolism in Cupriavidus basilensis 4G11

Best path

lctP, glcD, glcE, glcF

Also see fitness data for the top candidates

Rules

Overview: D-lactate catabolism in GapMind is based on D-lactate dehydrogenases, which form pyruvate.

14 steps (10 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
lctP D-lactate:H+ symporter LctP or LidP RR42_RS21280 RR42_RS14610
glcD D-lactate dehydrogenase, FAD-linked subunit 1 (GlcD) RR42_RS17300 RR42_RS29885
glcE D-lactate dehydrogenase, FAD-linked subunit 2 (GlcE) RR42_RS17310 RR42_RS29880
glcF D-lactate dehydrogenase, FeS subunit GlcF RR42_RS17315 RR42_RS29875
Alternative steps:
D-LDH D-lactate dehydrogenase RR42_RS17285 RR42_RS06435
larD D,L-lactic acid transporter
lctB electron-transfer flavoprotein for D-lactate dehydrogenase (NAD+, ferredoxin), small subunit
lctC electron-transfer flavoprotein for D-lactate dehydrogenase (NAD+, ferredoxin), large subunit RR42_RS04575
lctD D-lactate dehydrogenase (NAD+, ferredoxin), lactate dehydrogenase component RR42_RS17285 RR42_RS26635
mctP D,L-lactic acid transporter
PGA1_c12640 D-lactate ABC transporter, ATP-binding component RR42_RS11135 RR42_RS16965
PGA1_c12650 D-lactate ABC transporter, permease component 1 RR42_RS20265 RR42_RS26440
PGA1_c12660 D-lactate ABC transporter, permease component 2 RR42_RS16975 RR42_RS07535
PGA1_c12670 D-lactate ABC transporter, substrate-binding component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory