GapMind for catabolism of small carbon sources

 

N-acetyl-D-glucosamine catabolism in Pseudomonas fluorescens GW456-L13

Best path

nagF, nagEcb, nagA, nagB

Also see fitness data for the top candidates

Rules

Overview: N-acetylglucosamine utilization in GapMind is based on MetaCyc pathways N-acetylglucosamine degradation I (link) and pathway II (link). These pathways differ in whether uptake and phosphorylation are performed by a PTS system or performed separately by a transporter and a kinase.

21 steps (12 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
nagF N-acetylglucosamine phosphotransferase system, E-I, Hpr, and EII-A components (NagF) PfGW456L13_4832 PfGW456L13_5075
nagEcb N-acetylglucosamine phosphotransferase system, EII-CB components PfGW456L13_4833
nagA N-acetylglucosamine 6-phosphate deacetylase PfGW456L13_4830
nagB glucosamine 6-phosphate deaminase (isomerizing) PfGW456L13_4831 PfGW456L13_686
Alternative steps:
crr N-acetylglucosamine phosphotransferase system, EII-A component Crr PfGW456L13_4832
nag3 N-acetylglucosamine transporter nag3/nag4
nagEcba N-acetylglucosamine phosphotransferase system, EII-CBA components PfGW456L13_4833
nagEIIA N-acetylglucosamine phosphotransferase system, EII-A component (PtsG/YpqE/GamP) PfGW456L13_4833 PfGW456L13_4832
nagK N-acetylglucosamine kinase PfGW456L13_1890
nagP N-acetylglucosamine transporter NagP
nagPcb N-acetylglucosamine phosphotransferase system, EII-CB component NagP PfGW456L13_4833
ngcE N-acetylglucosamine ABC transporter, substrate-binding component (NgcE)
ngcF N-acetylglucosamine ABC transporter, permease component 1 (NgcF)
ngcG N-acetylglucosamine ABC transporter, permease component 2 (NgcG)
ngt1 N-acetylglucosamine:H+ symporter Ngt1
ptsB N-acetylglucosamine-specific phosphotransferase system, EII-B component PtsB
ptsC N-acetylglucosamine phosphotransferase system, EII-C component PtsC PfGW456L13_4833
SMc02869 N-acetylglucosamine ABC transporter, ATPase component PfGW456L13_3039 PfGW456L13_1897
SMc02871 N-acetylglucosamine ABC transporter, permease component 2 PfGW456L13_1896
SMc02872 N-acetylglucosamine ABC transporter, permease component 1
SMc02873 N-acetylglucosamine ABC transporter, substrate-binding component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory