dctP, dctQ, dctM, udh, uxuL, gudD, kdgD, dopDH
Also see fitness data for the top candidates
Overview: Glucuronate utilization in GapMind is based on MetaCyc pathways D-glucuronate degradation II (oxidation of 5-keto-4-deoxyglucarate, link), a related pathway via 5-keto-4-deoxyglucarate aldolase (link), or degradation via fructuronate (link). GapMind also includes a variation on the oxidative pathway with a glucarolactonase, as in Pseudomonas putida. MetaCyc pathway I (via L-gulonate and xylitol, link) is not reported in prokaryotes and is not described here.
Or see definitions of steps
| Step | Description | Best candidate | 2nd candidate |
|---|---|---|---|
| dctP | D-glucuronate TRAP transporter, solute receptor component | Pf6N2E2_487 | Pf6N2E2_1301 |
| dctQ | D-glucuronate TRAP transporter, small permease component | Pf6N2E2_486 | |
| dctM | D-glucuronate TRAP transporter, large permease component | Pf6N2E2_485 | Pf6N2E2_1302 |
| udh | D-glucuronate dehydrogenase | Pf6N2E2_489 | Pf6N2E2_4908 |
| uxuL | D-glucaro-1,5-lactonase UxuL or UxuF | Pf6N2E2_488 | Pf6N2E2_5966 |
| gudD | D-glucarate dehydratase | Pf6N2E2_2116 | Pf6N2E2_692 |
| kdgD | 5-dehydro-4-deoxyglucarate dehydratase | Pf6N2E2_3299 | Pf6N2E2_2747 |
| dopDH | 2,5-dioxopentanonate dehydrogenase | Pf6N2E2_612 | Pf6N2E2_3298 |
| Alternative steps: | |||
| eda | 2-keto-3-deoxygluconate 6-phosphate aldolase | Pf6N2E2_2883 | Pf6N2E2_5976 |
| exuT | D-glucuronate:H+ symporter ExuT | ||
| garK | glycerate 2-kinase | Pf6N2E2_5836 | Pf6N2E2_460 |
| garL | 5-dehydro-4-deoxy-D-glucarate aldolase | Pf6N2E2_1103 | Pf6N2E2_1314 |
| garR | tartronate semialdehyde reductase | Pf6N2E2_5837 | Pf6N2E2_1929 |
| gci | D-glucaro-1,4-lactone cycloisomerase | Pf6N2E2_5977 | Pf6N2E2_1104 |
| kdgK | 2-keto-3-deoxygluconate kinase | Pf6N2E2_2046 | Pf6N2E2_629 |
| uxaC | D-glucuronate isomerase | ||
| uxuA | D-mannonate dehydratase | Pf6N2E2_5977 | |
| uxuB | D-mannonate dehydrogenase | Pf6N2E2_806 | |
Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory