GapMind for catabolism of small carbon sources

 

L-proline catabolism in Dechlorosoma suillum PS

Best path

HSERO_RS00870, HSERO_RS00885, HSERO_RS00890, HSERO_RS00895, HSERO_RS00900, put1, putA

Also see fitness data for the top candidates

Rules

Overview: Proline degradation in GapMind is based on MetaCyc pathway I via glutamate semialdehyde dehydrogenase (link) and pathway II via 5-aminopentanoate (link). (MetaCyc describes 5-aminopentanoate, also known as 5-aminovalerate, as a fermentative end product, but it is further degraded

53 steps (28 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
HSERO_RS00870 proline ABC transporter, substrate-binding component Dsui_0630 Dsui_2061
HSERO_RS00885 proline ABC transporter, permease component 1 Dsui_0629 Dsui_1110
HSERO_RS00890 proline ABC transporter, permease component 2 Dsui_0628 Dsui_1111
HSERO_RS00895 proline ABC transporter, ATPase component 1 Dsui_0627 Dsui_2058
HSERO_RS00900 proline ABC transporter, ATPase component 2 Dsui_0626 Dsui_2057
put1 proline dehydrogenase
putA L-glutamate 5-semialdeyde dehydrogenase Dsui_0437 Dsui_1464
Alternative steps:
aapJ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), substrate-binding component AapJ
aapM ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM) Dsui_0637
aapP ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP Dsui_0638 Dsui_2068
aapQ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ)
AAT20.2 proline transporter
atoB acetyl-CoA C-acetyltransferase Dsui_0976 Dsui_3239
AZOBR_RS08235 proline ABC transporter, permease component 1 Dsui_0629 Dsui_1110
AZOBR_RS08240 proline ABC transporter, permease component 2 Dsui_0628 Dsui_2059
AZOBR_RS08245 proline ABC transporter, ATPase component 1 Dsui_0627 Dsui_2058
AZOBR_RS08250 proline ABC transporter, ATPase component 2 Dsui_2057 Dsui_0626
AZOBR_RS08260 proline ABC transporter, substrate-binding component Dsui_0630
BAC2 basic amino acid carrier BAC2
betS proline transporter BetS
CCNA_00435 proline transporter
davD glutarate semialdehyde dehydrogenase Dsui_0437 Dsui_0105
davT 5-aminovalerate aminotransferase Dsui_3250 Dsui_0023
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase Dsui_1378 Dsui_0323
ectP proline transporter EctP
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase Dsui_0318 Dsui_2330
gcdG succinyl-CoA:glutarate CoA-transferase Dsui_0037 Dsui_1115
gcdH glutaryl-CoA dehydrogenase Dsui_3369 Dsui_0977
glaH glutarate 2-hydroxylase, succinate-releasing (GlaH or CsiD)
hutV proline ABC transporter, ATPase component HutV Dsui_2943 Dsui_0258
hutW proline ABC transporter, permease component HutW
hutX proline ABC transporter, substrate-binding component HutX
lhgD L-2-hydroxyglutarate dehydrogenase or oxidase (LhgD or LhgO) Dsui_2329
N515DRAFT_2924 proline transporter
natA proline ABC transporter, ATPase component 1 (NatA) Dsui_2058 Dsui_0627
natB proline ABC transporter, substrate-binding component NatB
natC proline ABC transporter, permease component 1 (NatC) Dsui_0628
natD proline ABC transporter, permease component 2 (NatD) Dsui_0629 Dsui_2060
natE proline ABC transporter, ATPase component 2 (NatE) Dsui_0841 Dsui_2057
opuBA proline ABC transporter, ATPase component OpuBA/BusAA Dsui_0258 Dsui_3464
opuBB proline ABC transporter, fused permease and substrate-binding components OpuBB/BusAB
prdA D-proline reductase, prdA component
prdB D-proline reductase, prdB component
prdC D-proline reductase, electron transfer component PrdC
prdF proline racemase
proP proline:H+ symporter ProP
PROT1 proline transporter
proV proline ABC transporter, ATPase component ProV Dsui_0258 Dsui_1833
proW proline ABC transporter, permease component ProW
proX proline ABC transporter, substrate-binding component ProX
proY proline:H+ symporter
putP proline:Na+ symporter
SLC6A7 proline:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory