GapMind for catabolism of small carbon sources

 

L-proline catabolism in Shewanella loihica PV-4

Best path

putP, put1, putA

Also see fitness data for the top candidates

Rules

Overview: Proline degradation in GapMind is based on MetaCyc pathway I via glutamate semialdehyde dehydrogenase (link) and pathway II via 5-aminopentanoate (link). (MetaCyc describes 5-aminopentanoate, also known as 5-aminovalerate, as a fermentative end product, but it is further degraded

53 steps (28 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
putP proline:Na+ symporter Shew_3013 Shew_2435
put1 proline dehydrogenase Shew_0615
putA L-glutamate 5-semialdeyde dehydrogenase Shew_0615 Shew_3574
Alternative steps:
aapJ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), substrate-binding component AapJ
aapM ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM)
aapP ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP Shew_3164 Shew_0974
aapQ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ)
AAT20.2 proline transporter
atoB acetyl-CoA C-acetyltransferase Shew_1667 Shew_0018
AZOBR_RS08235 proline ABC transporter, permease component 1 Shew_2609
AZOBR_RS08240 proline ABC transporter, permease component 2
AZOBR_RS08245 proline ABC transporter, ATPase component 1 Shew_2611 Shew_3304
AZOBR_RS08250 proline ABC transporter, ATPase component 2 Shew_2606 Shew_3310
AZOBR_RS08260 proline ABC transporter, substrate-binding component
BAC2 basic amino acid carrier BAC2
betS proline transporter BetS Shew_0049
CCNA_00435 proline transporter
davD glutarate semialdehyde dehydrogenase Shew_3173 Shew_0967
davT 5-aminovalerate aminotransferase Shew_3172 Shew_0578
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase Shew_1670 Shew_0019
ectP proline transporter EctP Shew_0049
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase Shew_0019 Shew_2425
gcdG succinyl-CoA:glutarate CoA-transferase
gcdH glutaryl-CoA dehydrogenase Shew_0900 Shew_2570
glaH glutarate 2-hydroxylase, succinate-releasing (GlaH or CsiD)
HSERO_RS00870 proline ABC transporter, substrate-binding component
HSERO_RS00885 proline ABC transporter, permease component 1 Shew_2609
HSERO_RS00890 proline ABC transporter, permease component 2 Shew_2608
HSERO_RS00895 proline ABC transporter, ATPase component 1 Shew_2611 Shew_3310
HSERO_RS00900 proline ABC transporter, ATPase component 2 Shew_2606 Shew_3310
hutV proline ABC transporter, ATPase component HutV Shew_0974 Shew_0863
hutW proline ABC transporter, permease component HutW
hutX proline ABC transporter, substrate-binding component HutX
lhgD L-2-hydroxyglutarate dehydrogenase or oxidase (LhgD or LhgO) Shew_0530
N515DRAFT_2924 proline transporter
natA proline ABC transporter, ATPase component 1 (NatA) Shew_2611 Shew_3310
natB proline ABC transporter, substrate-binding component NatB
natC proline ABC transporter, permease component 1 (NatC)
natD proline ABC transporter, permease component 2 (NatD) Shew_2609
natE proline ABC transporter, ATPase component 2 (NatE) Shew_2606 Shew_3164
opuBA proline ABC transporter, ATPase component OpuBA/BusAA Shew_0974 Shew_0094
opuBB proline ABC transporter, fused permease and substrate-binding components OpuBB/BusAB
prdA D-proline reductase, prdA component
prdB D-proline reductase, prdB component
prdC D-proline reductase, electron transfer component PrdC
prdF proline racemase Shew_2363 Shew_2361
proP proline:H+ symporter ProP
PROT1 proline transporter
proV proline ABC transporter, ATPase component ProV Shew_0974 Shew_3164
proW proline ABC transporter, permease component ProW
proX proline ABC transporter, substrate-binding component ProX
proY proline:H+ symporter
SLC6A7 proline:Na+ symporter Shew_2896

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory